MAP4K2 antibody

Catalog No. ABIN7116461

The Rabbit Polyclonal anti-MAP4K2 antibody is suitable to detect MAP4K2 in samples from Human, Mouse and Rat. It has been validated for WB and ELISA.

Quick Overview for MAP4K2 antibody (ABIN7116461)

Target: MAP4K2 (Mitogen-Activated Protein Kinase Kinase Kinase Kinase 2 (MAP4K2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAP4K2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-MAP4K2 Antibody

Purpose: MAP4K2 antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: mitogen-activated protein kinase kinase kinase kinase 2

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for MAP4K2

Target: MAP4K2 (Mitogen-Activated Protein Kinase Kinase Kinase Kinase 2 (MAP4K2))

Alternative Name: MAP4K2

Background:

Synonyms: Mitogen-activated protein kinase kinase kinase kinase 2|B lymphocyte serine/threonine-protein kinase|Germinal center kinase (GC kinase)|MAPK/ERK kinase kinase kinase 2 (MEK kinase kinase 2, MEKKK 2)|Rab8-interacting protein|MAP4K2|GCK|RAB8IP

Background: Serine/threonine-protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Acts as a MAPK kinase kinase kinase(MAP4K) and is an upstream activator of the stress-activated protein kinase/c-Jun N-terminal kinase(SAP/JNK) signaling pathway and to a lesser extend of the p38 MAPKs signaling pathway. Required for the efficient activation of JNKs by TRAF6-dependent stimuli, including pathogen-associated molecular patterns(PAMPs) such as polyinosine-polycytidine(poly(IC)), lipopolysaccharides(LPS), lipid A, peptidoglycan(PGN), or bacterial flagellin. To a lesser degree, IL-1 and engagement of CD40 also stimulate MAP4K2-mediated JNKs activation. The requirement for MAP4K2/GCK is most pronounced for LPS signaling, and extends to LPS stimulation of c-Jun phosphorylation and induction of IL-8. Enhances MAP3K1 oligomerization, which may relieve N-terminal mediated MAP3K1 autoinhibition and lead to activation following autophosphorylation. Mediates also the SAP/JNK signaling pathway and the p38 MAPKs signaling pathway through activation of the MAP3Ks MAP3K10/MLK2 and MAP3K11/MLK3. May play a role in the regulation of vesicle targeting or fusion. regulation of vesicle targeting or fusion.

Molecular Weight: 85-91 kDa, 58 kDa

Gene ID: 5871

UniProt: Q12851