MCC antibody

Catalog No. ABIN7116539

The Rabbit Polyclonal anti-MCC antibody has been validated for ELISA, WB, IHC, IP and IF. It is suitable to detect MCC in samples from Human and Mouse.

Quick Overview for MCC antibody (ABIN7116539)

Target: MCC (Mutated in Colorectal Cancers (MCC))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MCC antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)

Product Details anti-MCC Antibody

Purpose: MCC antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: mutated in colorectal cancers

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:2000, IP: 1:200-1:1000, IHC: 1:20-1:200, IF: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for MCC

Target: MCC (Mutated in Colorectal Cancers (MCC))

Alternative Name: MCC

Background:

Synonyms: Colorectal mutant cancer protein (Protein MCC)|MCC

Background: Candidate for the putative colorectal tumor suppressor gene located at 5q21. Suppresses cell proliferation and the Wnt/b-catenin pathway in colorectal cancer cells. Inhibits DNA binding of b-catenin/TCF/LEF transcription factors. Involved in cell migration independently of RAC1, CDC42 and p21-activated kinase(PAK) activation(PubMed:18591935, PubMed:19555689, PubMed:22480440). Represses the beta-catenin pathway(canonical Wnt signaling pathway) in a CCAR2-dependent manner by sequestering CCAR2 to the cytoplasm, thereby impairing its ability to inhibit SIRT1 which is involved in the deacetylation and negative regulation of beta-catenin(CTNB1) transcriptional activity(PubMed:24824780).

Molecular Weight: 98 kDa,113-118 kDa

Gene ID: 4163

UniProt: P23508