NPL antibody

Catalog No. ABIN7116673

This Rabbit Polyclonal antibody specifically detects NPL in WB, ELISA and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for NPL antibody (ABIN7116673)

Target: NPL (N-Acetylneuraminate Pyruvate Lyase (Dihydrodipicolinate Synthase) (NPL))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NPL antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Product Details anti-NPL Antibody

Purpose: NPL antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: N-acetylneuraminate pyruvate lyase(dihydrodipicolinate synthase)

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:2000, IF: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for NPL

Target: NPL (N-Acetylneuraminate Pyruvate Lyase (Dihydrodipicolinate Synthase) (NPL))

Alternative Name: NPL

Background:

Synonyms: N-acetylneuraminate lyase (NALase)|N-acetylneuraminate pyruvate-lyase|N-acetylneuraminic acid aldolase|Sialate lyase|Sialate-pyruvate lyase|Sialic acid aldolase|Sialic acid lyase|NPL|C1orf13

Background: Catalyzes the cleavage of N-acetylneuraminic acid(sialic acid) to form pyruvate and N-acetylmannosamine via a Schiff base intermediate. It prevents sialic acids from being recycled and returning to the cell surface. Involved in the N-glycolylneuraminic acid(Neu5Gc) degradation pathway. Although human is not able to catalyze formation of Neu5Gc due to the inactive CMAHP enzyme, Neu5Gc is present in food and must be degraded(By similarity).

Molecular Weight: 35 kDa

Gene ID: 80896

UniProt: Q9BXD5