NDC80 antibody

Catalog No. ABIN7116759

The Rabbit Polyclonal anti-NDC80 antibody (ABIN7116759) specifically detects NDC80 in WB, ELISA, IP and IHC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for NDC80 antibody (ABIN7116759)

Target: NDC80 (NDC80 Kinetochore Complex Component Homolog (NDC80))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NDC80 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (IHC)

Product Details anti-NDC80 Antibody

Purpose: NDC80 antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: NDC80 homolog, kinetochore complex component

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:2000, IP: 1:200-1:1000, IHC: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for NDC80

Target: NDC80 (NDC80 Kinetochore Complex Component Homolog (NDC80))

Alternative Name: NDC80

Background:

Synonyms: Kinetochore protein NDC80 homolog|Highly expressed in cancer protein|Kinetochore protein Hec1 (HsHec1)|Kinetochore-associated protein 2|Retinoblastoma-associated protein HEC|NDC80|HEC|HEC1|KNTC2

Background: Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity(PubMed:9315664, PubMed:12351790, PubMed:14654001, PubMed:14699129, PubMed:15062103, PubMed:15235793, PubMed:15239953, PubMed:15548592, PubMed:16732327). Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore(PubMed:15548592). The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules(PubMed:23085020).

Molecular Weight: 76 kDa

Gene ID: 10403

UniProt: O14777

Pathways: Maintenance of Protein Location