Nestin antibody

Catalog No. ABIN7116782

The Mouse Monoclonal anti-Nestin antibody has been validated for IHC, WB, ELISA and IF. It is suitable to detect Nestin in samples from Human, Mouse and Rat.

Quick Overview for Nestin antibody (ABIN7116782)

Target: Nestin (NES)

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Nestin antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), ELISA, Immunofluorescence (IF)

Clone: 4F9

Product Details anti-Nestin Antibody

Purpose: NES antibody

Purification: Protein A+G purification

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: nestin

Isotype: IgG1

Application Details

Application Notes: WB: 1:500-1:2000, IHC: 1:20-1:1000, IF: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for Nestin

Target: Nestin (NES)

Alternative Name: NES

Background:

Synonyms: Nestin|NES

Background: Nestin, also named as NES and Nbla00170, is a class VI intermediate filament protein expressed in stem cells of the central nervous system(CNS) but not in mature CNS cells. Its expression is used extensively as a marker for neural lineage cells derived from human embryonic stem(hES) and induced pluripotent stem(iPS) cells. Nestin is also expressed in non-neural stem cell populations such as pancreatic islet progenitors and hematopoietic progenitors.

Molecular Weight: 170 kDa

Gene ID: 10763

UniProt: P48681