NEK2 antibody

Catalog No. ABIN7116883

This Rabbit Polyclonal antibody specifically detects NEK2 in WB, ELISA and IHC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for NEK2 antibody (ABIN7116883)

Target: NEK2 (NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NEK2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-NEK2 Antibody

Purpose: NEK2 antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: NIMA(never in mitosis gene a)-related kinase 2

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:2000, IHC: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for NEK2

Target: NEK2 (NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2))

Alternative Name: NEK2

Background:

Synonyms: Serine/threonine-protein kinase Nek2|HSPK 21|Never in mitosis A-related kinase 2 (NimA-related protein kinase 2)|NimA-like protein kinase 1|NEK2|NEK2A|NLK1

Background: Protein kinase which is involved in the control of centrosome separation and bipolar spindle formation in mitotic cells and chromatin condensation in meiotic cells. Regulates centrosome separation(essential for the formation of bipolar spindles and high-fidelity chromosome separation) by phosphorylating centrosomal proteins such as CROCC, CEP250 and NINL, resulting in their displacement from the centrosomes. Regulates kinetochore microtubule attachment stability in mitosis via phosphorylation of NDC80. Involved in regulation of mitotic checkpoint protein complex via phosphorylation of CDC20 and MAD2L1. Plays an active role in chromatin condensation during the first meiotic division through phosphorylation of HMGA2. Phosphorylates: PPP1CC, SGO1, NECAB3 and NPM1. Essential for localization of MAD2L1 to kinetochore and MAPK1 and NPM1 to the centrosome. Phosphorylates CEP68 and CNTLN directly or indirectly(PubMed:24554434). NEK2-mediated phosphorylation of CEP68 promotes CEP68 dissociation from the centrosome and its degradation at the onset of mitosis(PubMed:25704143). Involved in the regulation of centrosome disjunction(PubMed:26220856). Isoform 1: Phosphorylates and activates NEK11 in G1/S-arrested cells. Isoform 2: Not present in the nucleolus and, in contrast to isoform 1, does not phosphorylate and activate NEK11 in G1/S-arrested cells.

Molecular Weight: 48 kDa

UniProt: P51955

Pathways: M Phase