OSBP antibody

Catalog No. ABIN7117171

This Rabbit Polyclonal antibody specifically detects OSBP in WB, ELISA, IHC and IP. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for OSBP antibody (ABIN7117171)

Target: OSBP (Oxysterol Binding Protein (OSBP))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This OSBP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

Product Details anti-OSBP Antibody

Purpose: OSBP antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: oxysterol binding protein

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:2000, IP: 1:200-1:1000, IHC: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for OSBP

Target: OSBP (Oxysterol Binding Protein (OSBP))

Alternative Name: OSBP

Background:

Synonyms: Oxysterol-binding protein 1|OSBP|OSBP1

Background: Lipid transporter involved in lipid countertransport between the Golgi complex and membranes of the endoplasmic reticulum: specifically exchanges sterol with phosphatidylinositol 4-phosphate(PI4P), delivering sterol to the Golgi in exchange for PI4P, which is degraded by the SAC1/SACM1L phosphatase in the endoplasmic reticulum(PubMed:24209621). Binds cholesterol and a range of oxysterols including 25-hydroxycholesterol(PubMed:15746430, PubMed:17428193). Cholesterol binding promotes the formation of a complex with PP2A and a tyrosine phosphatase which dephosphorylates ERK1/2, whereas 25-hydroxycholesterol causes its disassembly(PubMed:15746430). Regulates cholesterol efflux by decreasing ABCA1 stability(PubMed:18450749).

Molecular Weight: 89 kDa

Gene ID: 5007

UniProt: P22059