POLR3G antibody

Catalog No. ABIN7117683

This anti-POLR3G antibody is a Rabbit Polyclonal antibody detecting POLR3G in WB and ELISA. Suitable for Human.

Quick Overview for POLR3G antibody (ABIN7117683)

Target: POLR3G (Polymerase (RNA) III (DNA Directed) Polypeptide G (32kD) (POLR3G))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLR3G antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-POLR3G Antibody

Purpose: POLR3G antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: polymerase(RNA) III(DNA directed) polypeptide G(32kD)

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for POLR3G

Target: POLR3G (Polymerase (RNA) III (DNA Directed) Polypeptide G (32kD) (POLR3G))

Alternative Name: POLR3G

Background:

Synonyms: DNA-directed RNA polymerase III subunit RPC7 (RNA polymerase III subunit C7)|DNA-directed RNA polymerase III subunit G|RNA polymerase III 32 kDa apha subunit (RPC32-alpha)|RNA polymerase III 32 kDa subunit (RPC32)|POLR3G

Background: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. May direct with other members of the RPC3/POLR3C-RPC6/POLR3F-RPC7/POLR3G subcomplex RNA Pol III binding to the TFIIIB-DNA complex via the interactions between TFIIIB and POLR3F. May be involved either in the recruitment and stabilization of the subcomplex within RNA polymerase III, or in stimulating catalytic functions of other subunits during initiation. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs(EBERs) induce type I interferon and NF-Kappa-B through the RIG-I pathway.

Molecular Weight: 26-30 kDa

Gene ID: 10622

UniProt: O15318