ErbB2/Her2 antibody

Catalog No. ABIN7118347

This Rabbit Polyclonal antibody specifically detects ErbB2/Her2 in WB, IHC, ELISA, FACS and IP. It exhibits reactivity toward Human and Mouse.

Quick Overview for ErbB2/Her2 antibody (ABIN7118347)

Target: ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ErbB2/Her2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunoprecipitation (IP)

Product Details anti-ErbB2/Her2 Antibody

Purpose: ERBB2 antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog(avian)

Isotype: IgG

Application Details

Application Notes: WB: 1:1000-1:10000, IP: 1:200-1:2000, IHC: 1:50-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for ErbB2/Her2

Target: ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))

Alternative Name: ERBB2

Background:

Synonyms: Receptor tyrosine-protein kinase erbB-2|Metastatic lymph node gene 19 protein (MLN 19)|Proto-oncogene Neu|Proto-oncogene c-ErbB-2|Tyrosine kinase-type cell surface receptor HER2|p185erbB2|ERBB2|HER2|MLN19|NEU|NGL

Background: HER2, also known as ErbB2 and Neu, is a 185- kDa transmembrane glycoprotein that is a member of the epidermal growth factor(EGF) receptor family of receptor tyrosine kinases. It has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Amplification and/or overexpression of HER2 have been reported in numerous cancers, including breast and ovarian tumors. HER2 is a therapeutic target for the treatment of breast cancer and other carcinomas. This antibody raised against a synthesized peptide corresponding to 1237-1255aa of human HER2 recognizes the 185- kDa full-length glycosylated form and other lower molecular-mass forms of HER2, including the truncated form.

Molecular Weight: 185 kDa

Gene ID: 2064

UniProt: P04626

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Skeletal Muscle Fiber Development