ROCK2 antibody

Catalog No. ABIN7118500

This Rabbit Polyclonal antibody specifically detects ROCK2 in WB, ELISA, IHC, IP and IF. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for ROCK2 antibody (ABIN7118500)

Target: ROCK2 (rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ROCK2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)

Product Details anti-ROCK2 Antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: Rho-associated, coiled-coil containing protein kinase 2

Isotype: IgG

Application Details

Application Notes: WB: 1:1000-1:10000, IP: 1:200-1:2000, IHC: 1:20-1:200, IF: 1:10-1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

Expiry Date: 12 months

Target Details for ROCK2

Target: ROCK2 (rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2))

Alternative Name: ROCK2

Background: Synonyms:KIAA0619 Background:Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of ADD1, BRCA2, CNN1, EZR, DPYSL2, EP300, MSN, MYL9/MLC2, NPM1, RDX, PPP1R12A and VIM. Phosphorylates SORL1 and IRF4. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Positively regulates the activation of p42/MAPK1-p44/MAPK3 and of p90RSK/RPS6KA1 during myogenic differentiation. Plays an important role in the timely initiation of centrosome duplication. Inhibits keratinocyte terminal differentiation. May regulate closure of the eyelids and ventral body wall through organization of actomyosin bundles. Plays a critical role in the regulation of spine and synaptic properties in the hippocampus. Plays an important role in generating the circadian rhythm of the aortic myofilament Ca(2+) sensitivity and vascular contractility by modulating the myosin light chain phosphorylation.

Molecular Weight: 161 kDa

Gene ID: 9475

UniProt: O75116

Pathways: Microtubule Dynamics, WNT Signaling, Tube Formation