RNF20 antibody

Catalog No. ABIN7118646

The Rabbit Polyclonal anti-RNF20 antibody (ABIN7118646) specifically detects RNF20 in WB, ELISA, IF and IP. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for RNF20 antibody (ABIN7118646)

Target: RNF20 (Ring Finger Protein 20 (RNF20))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RNF20 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP)

Product Details anti-RNF20 Antibody

Purpose: RNF20 antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: ring finger protein 20

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:2000, IP: 1:200-1:1000, IF: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for RNF20

Target: RNF20 (Ring Finger Protein 20 (RNF20))

Alternative Name: RNF20

Background:

Synonyms: E3 ubiquitin-protein ligase BRE1A (BRE1-A, hBRE1)|RING finger protein 20|RING-type E3 ubiquitin transferase BRE1A|RNF20|BRE1A

Background: Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B(H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation(H3K4me and H3K79me, respectively). It thereby plays a central role inb histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B, reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. Recruited to the MDM2 promoter, probably by being recruited by p53/TP53, and thereby acts as a transcriptional coactivator. Mediates the polyubiquitination of isoform 2 of PA2G4 in cancer cells leading to its proteasome-mediated degradation.

Molecular Weight: 114 kDa

Gene ID: 56254

UniProt: Q5VTR2