RNF4 antibody

Catalog No. ABIN7118657

The Rabbit Polyclonal anti-RNF4 antibody has been validated for ELISA and IP. It is suitable to detect RNF4 in samples from Human, Mouse and Rat.

Quick Overview for RNF4 antibody (ABIN7118657)

Target: RNF4 (Ring Finger Protein 4 (RNF4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RNF4 antibody is un-conjugated

Application: ELISA, Immunoprecipitation (IP)

Product Details anti-RNF4 Antibody

Purpose: RNF4 antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: ring finger protein 4

Isotype: IgG

Application Details

Application Notes: IP: 1:200-1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for RNF4

Target: RNF4 (Ring Finger Protein 4 (RNF4))

Alternative Name: RNF4

Background:

Synonyms: E3 ubiquitin-protein ligase RNF4|RING finger protein 4|Small nuclear ring finger protein (Protein SNURF)|RNF4|SNURF

Background: E3 ubiquitin-protein ligase which binds polysumoylated chains covalently attached to proteins and mediates 'Lys-6'-, 'Lys-11'-, 'Lys-48'-and 'Lys-63'-linked polyubiquitination of those substrates and their subsequent targeting to the proteasome for degradation. Regulates the degradation of several proteins including PML and the transcriptional activator PEA3. Involved in chromosome alignment and spindle assembly, it regulates the kinetochore CENPH-CENPI-CENPK complex by targeting polysumoylated CENPI to proteasomal degradation. Regulates the cellular responses to hypoxia and heat shock through degradation of respectively EPAS1 and PARP1. Alternatively, it may also bind DNA/nucleosomes and have a more direct role in the regulation of transcription for instance enhancing basal transcription and steroid receptor-mediated transcriptional activation.

Gene ID: 6047

UniProt: P78317

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway