RBM25 antibody

Catalog No. ABIN7118681

The Rabbit Polyclonal anti-RBM25 antibody is suitable to detect RBM25 in samples from Mouse and Human. It has been validated for WB, ELISA and IHC.

Quick Overview for RBM25 antibody (ABIN7118681)

Target: RBM25 (RNA Binding Motif Protein 25 (RBM25))

Reactivity: Mouse, Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RBM25 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-RBM25 Antibody

Purpose: RBM25 antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: RNA binding motif protein 25

Isotype: IgG

Application Details

Application Notes: WB: 1:200-1:2000, IP: 1:200-1:2000, IHC: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for RBM25

Target: RBM25 (RNA Binding Motif Protein 25 (RBM25))

Alternative Name: RBM25

Background:

Synonyms: RNA-binding protein 25|Arg/Glu/Asp-rich protein of 120 kDa (RED120)|Protein S164|RNA-binding motif protein 25|RNA-binding region-containing protein 7|RBM25|RNPC7

Background: RNA-binding protein that acts as a regulator of alternative pre-mRNA splicing. Involved in apoptotic cell death through the regulation of the apoptotic factor BCL2L1 isoform expression. Modulates the ratio of proapoptotic BCL2L1 isoform S to antiapoptotic BCL2L1 isoform L mRNA expression. When overexpressed, stimulates proapoptotic BCL2L1 isoform S 5'-splice site(5'-ss) selection, whereas its depletion caused the accumulation of antiapoptotic BCL2L1 isoform L. Promotes BCL2L1 isoform S 5'-ss usage through the 5'-CGGGCA-3' RNA sequence. Its association with LUC7L3 promotes U1 snRNP binding to a weak 5' ss in a 5'-CGGGCA-3'-dependent manner. Binds to the exonic splicing enhancer 5'-CGGGCA-3' RNA sequence located within exon 2 of the BCL2L1 pre-mRNA. Also involved in the generation of an abnormal and truncated splice form of SCN5A in heart failure.

Molecular Weight: 110-120 kDa

Gene ID: 58517

UniProt: P49756