RBM4 antibody

Catalog No. ABIN7118687

The Mouse Monoclonal anti-RBM4 antibody is suitable to detect RBM4 in samples from Human. It has been validated for WB, ELISA, IHC and IF.

Quick Overview for RBM4 antibody (ABIN7118687)

Target: RBM4 (RNA Binding Motif Protein 4 (RBM4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This RBM4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: 6F6

Product Details anti-RBM4 Antibody

Purpose: RBM4 antibody

Purification: Protein A+G purification

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: RNA binding motif protein 4

Isotype: IgG1

Application Details

Application Notes: WB: 1:500-1:2000, IHC: 1:20-1:200, IF: 1:50-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for RBM4

Target: RBM4 (RNA Binding Motif Protein 4 (RBM4))

Alternative Name: RBM4

Background:

Synonyms: RNA-binding protein 4|Lark homolog (hLark)|RNA-binding motif protein 4|RNA-binding motif protein 4a|RBM4|RBM4A

Background: RNA-binding factor involved in multiple aspects of cellular processes like alternative splicing of pre-mRNA and translation regulation. Modulates alternative 5'-splice site and exon selection. Acts as a muscle cell differentiation-promoting factor. Activates exon skipping of the PTB pre-mRNA during muscle cell differentiation. Antagonizes the activity of the splicing factor PTBP1 to modulate muscle cell-specific exon selection of alpha tropomyosin. Binds to intronic pyrimidine-rich sequence of the TPM1 and MAPT pre-mRNAs. Required for the translational activation of PER1 mRNA in response to circadian clock. Binds directly to the 3'-UTR of the PER1 mRNA. Exerts a suppressive activity on Cap-dependent translation via binding to CU-rich responsive elements within the 3'UTR of mRNAs, a process increased under stress conditions or during myocytes differentiation. Recruits EIF4A1 to stimulate IRES-dependent translation initiation in respons to cellular stress. Associates to internal ribosome entry segment(IRES) in target mRNA species under stress conditions. Plays a role for miRNA-guided RNA cleavage and translation suppression by promoting association of AGO2-containing miRNPs with their cognate target mRNAs. Associates with miRNAs during muscle cell differentiation. Binds preferentially to 5'-CGCGCG[GCA]-3' motif in vitro.

Molecular Weight: 40 kDa

Gene ID: 5936

UniProt: Q9BWF3

Pathways: Regulation of Muscle Cell Differentiation, Photoperiodism