AQP2 antibody (Aquaporin 2 (Collecting Duct)) (AA 177-202)

Details for Product anti-AQP2 Antibody No. ABIN707576
Antigen
  • AQP-CD
  • WCH-CD
  • cph
  • jpk
  • AQP-2
  • aquaporin-2
  • aquaporin 2
  • aquaporin 2 (collecting duct)
  • Aqp2
  • AQP2
Alternatives
anti-Mouse (Murine) AQP2 antibody for ELISA
Epitope
AA 177-202
64
26
22
21
21
15
15
15
12
4
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
212
204
189
17
11
10
5
4
3
3
2
2
1
1
Host
Rabbit
262
6
1
Clonality
Polyclonal
Conjugate
This AQP2 antibody is un-conjugated
17
12
10
7
7
7
7
7
6
6
6
6
6
5
4
3
3
3
3
3
3
3
3
3
3
3
3
2
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
163
94
76
63
60
32
26
25
23
7
6
2
1
1
Options
'Independent Validation' Badge
Lot Number 999994W
Method validated Immunohistochemistry
Positive Control - Human kidney tubules - Human breast ductal epithelium
Negative Control - Human kidney glomeruli and stromal tissue - Human breast mesenchymal tissue
Notes Strong signal was observed in positive control tissues and not in negative control tissues.
Primary Antibody
  • Antibody: Aquaporin 2
  • Catalog number: ABIN707576
  • Batch number: 999994W
Isotype Control Antibody
  • Antibody: Rabbit IgG isotype control
  • Lot number: C11487
Secondary Antibody
  • Antibody: Biotinylated goat anti-rabbit/anti-mouse (Kit)
  • Lot number: D05923BA
Controls
  • Tissues stained came from a human FFPE tissue microarray (12-003d).
  • Positive control: Ductal epithelium from human kidney and breast tissue (specimen known to contain the target protein).
  • Negative Control: Stroma/mesenchyme from human kidney and breast tissue (specimen known to not contain the target protein).
  • Primary antibody isotype control: Ductal epithelium from human kidney and breast tissue (specimen known to contain the target protein) treated with primary antibody isotype control instead of the primary antibody.
  • Secondary antibody only control: Ductal epithelium from human kidney and breast tissue (specimen known to contain the target protein) treated with secondary antibody only (no primary antibody).
Protocol
  • Immunohistochemistry was performed on a Ventana NexES automated platform, instrument manufacturer specific reagents are italicized.
  • 1. Slides were preheated in convection oven at 60°C for 30 min
  • 2. Deparaffinization procedure: - 3 changes of Xylene, 5 min each - 3 changes of 100% Ethanol, 3 min each - 3 changes of 95% Ethanol, 3 min each - Rinsed in distilled water, 3 changes
  • 3. Heat retrieval procedure - Slides retrieved in 10.0 mM Citrate, pH6.0 in a 1000W microwave oven (~100°C) for 15 min. - Slides were allowed to cool (in citrate) for 30 min. - Slides were washed x 3 in Distilled water
  • 4. NexES instrument procedure, iVIEW DAB paraffin protocol (*abridged*): - Slide chamber warmed to 37°C
  • 5. Slides rinsed with reaction buffer x3
  • 6. *iVIEW Inhibitor (H2O2)* applied and incubated for 4 min
  • 7. Slides rinsed with *reaction buffer*
  • 8. Antibody Application - Primary antibody diluted 1:250 in PBS (100 µL applied/slide) - Ventana Isotype control applied neat - Slides Incubated overnight at room temperature (~12 hours ~25°C)
  • 9. Slides rinsed with *reaction buffer* x3
  • 10. *iVIEW Biotinylated IgG* applied and incubated for 8 min
  • 11. Slides rinsed with *reaction buffer*
  • 12. *iVIEW Streptavidin-Horseradish Peroxidase* applied and incubated for 8 min
  • 13. Slides rinsed with *reaction buffer*
  • 14. *iVIEW DAB/H2O2* applied and incubated for 8 min
  • 15. Slides rinsed with *reaction buffer*
  • 16. *iVIEW Copper* applied and incubated for 4 min
  • 17. Slides rinsed with *reaction buffer*
  • 18. Slides washed in Dawn Detergent/tap water
  • 19. Counterstain Procedure - Hematoxylin (Leica 560 MX) 30 seconds - Slides washed in tap water, 1 min - Decolorized (10% Acetic Acid in 70% ethanol), 1 min - Slides washed in tap water, 1 min - Bluing (Austin Clear Ammonia), 1 min - Slides washed in tap water, 1 min
  • 20. Dehydration/coverslipping procedure: - 3 changes of 95% Ethanol, 3 min each - 3 changes of 100% Ethanol, 3 min each - 3 changes of Xylene, 5 min each - Mounted with Permount
  • 21. Imaging: Leica SCN 400F Whole Slide Scanner with Digital Image Hub and Leica Slidepath software
Experimental Notes
  • Deviations from protocol/procedure supplied by manufacturer.
  • Step 1: Heated tissue 60°C for 30 minutes; manufacturer heats for 45 minutes.
  • Step 2: No ethanol wash was performed during deparaffinization; manufacturer includes 1 wash of 80% ethanol for 3 minutes.
  • Step 3.1: Slides heated for 15 minutes; manufacturer provides a range of 15-20 minutes.
  • Step 3.2: Slides cooled for 30 minutes; manufacturer cools for 20 minutes.
  • Step 4: Italicized reagents and incubation time are fixed instrument parameters.
  • Step 5: Secondary species-specific serum block not used; manufacturer blocks with 5% normal goat serum for 2 hours.
Validation Images
Immunohistochemistry validation image for anti-Aquaporin 2 (Collecting Duct) (AQP2) (AA 177-202) antibody (ABIN707576) Figure 1: Human kidney stained with AQP2 (brown).
Immunohistochemistry validation image for anti-Aquaporin 2 (Collecting Duct) (AQP2) (AA 177-202) antibody (ABIN707576) Figure 2. Human kidney stained with isotype control antibody (brown).
Immunohistochemistry validation image for anti-Aquaporin 2 (Collecting Duct) (AQP2) (AA 177-202) antibody (ABIN707576) Figure 3: Human kidney stained only with secondary antibody (primary antibody omitted...
Immunohistochemistry validation image for anti-Aquaporin 2 (Collecting Duct) (AQP2) (AA 177-202) antibody (ABIN707576) Figure 4: Human breast stained with AQP2 (brown).
Immunohistochemistry validation image for anti-Aquaporin 2 (Collecting Duct) (AQP2) (AA 177-202) antibody (ABIN707576) Figure 5. Human breast stained with isotype control antibody (brown).
Immunohistochemistry validation image for anti-Aquaporin 2 (Collecting Duct) (AQP2) (AA 177-202) antibody (ABIN707576) Figure 6: Human breast stained only with secondary antibody (primary antibody omitted...
Immunogen KLH conjugated synthetic peptide derived from human AQP2.
Isotype IgG
Purification Purified by Protein A.
Plasmids, Primers & others Plasmids, Primers & others AQP2 products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name Aqp2 (AQP2 Antibody Abstract)
Background

Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.

Subcellular location: Extracellular

Synonyms: AQP-CD, WCH-CD, Aquaporin-2, AQP-2, ADH water channel, Aquaporin-CD, Collecting duct water channel protein, Water channel protein for renal collecting duct, AQP2

Gene ID 359
UniProt P41181
Pathways Response to Water Deprivation
Application Notes FCM 1:20-100
IHC-P 1:100-500
IF(IHC-P) 1:50-200
Restrictions For Research Use only
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months
Supplier Images
Immunohistochemistry (IHC) image for anti-Aquaporin 2 (Collecting Duct) (AQP2) (AA 177-202) antibody (ABIN707576) Independently Validated Antibody, image provided by Science Direct, badge number 0294...
Immunohistochemistry (IHC) image for anti-Aquaporin 2 (Collecting Duct) (AQP2) (AA 177-202) antibody (ABIN707576) Formalin-fixed and paraffin embedded mouse intestine labeled with Anti-AQP2 Polyclona...
Immunohistochemistry (IHC) image for anti-Aquaporin 2 (Collecting Duct) (AQP2) (AA 177-202) antibody (ABIN707576) Independently Validated Antibody, image provided by Science Direct, badge number 0294...
Western Blotting (WB) image for anti-Aquaporin 2 (Collecting Duct) (AQP2) (AA 177-202) antibody (ABIN707576) Mouse kidney lysates probed with Anti-AQP2 Polyclonal Antibody, Unconjugated at 1:50...
Flow Cytometry (FACS) image for anti-Aquaporin 2 (Collecting Duct) (AQP2) (AA 177-202) antibody (ABIN707576) Mouse kidney cells probed with Rabbit Anti-AQP2 Polyclonal Antibody FITC Conjugated ...
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) image for anti-Aquaporin 2 (Collecting Duct) (AQP2) (AA 177-202) antibody (ABIN707576) Formalin-fixed and paraffin embedded mouse kidney tissue labeled with Anti-AQP2 Polyc...
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