RWDD3 antibody

Catalog No. ABIN7118740

This Rabbit Polyclonal antibody specifically detects RWDD3 in ELISA and IHC. It exhibits reactivity toward Human.

Quick Overview for RWDD3 antibody (ABIN7118740)

Target: RWDD3 (RWD Domain Containing 3 (RWDD3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RWDD3 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Product Details anti-RWDD3 Antibody

Purpose: RWDD3 antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: RWD domain containing 3

Isotype: IgG

Application Details

Application Notes: IHC: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for RWDD3

Target: RWDD3 (RWD Domain Containing 3 (RWDD3))

Alternative Name: RWDD3

Background:

Synonyms: RWD domain-containing protein 3|RWD domain-containing sumoylation enhancer (RSUME)|RWDD3|RSUME

Background: Enhancer of SUMO conjugation. Via its interaction with UBE2I/UBC9, increases SUMO conjugation to proteins by promoting the binding of E1 and E2 enzymes, thioester linkage between SUMO and UBE2I/UBC9 and transfer of SUMO to specific target proteins which include HIF1A, PIAS, NFKBIA, NR3C1 and TOP1. Isoform 1 and isoform 2 positively regulate the NF-kappa-B signaling pathway by enhancing the sumoylation of NF-kappa-B inhibitor alpha(NFKBIA), promoting its stabilization which consequently leads to an increased inhibition of NF-kappa-B transcriptional activity. Isoform 1 and isoform 2 negatively regulate the hypoxia-inducible factor-1 alpha(HIF1A) signaling pathway by increasing the sumoylation of HIF1A, promoting its stabilization, transcriptional activity and the expression of its target gene VEGFA during hypoxia. Isoform 2 promotes the sumoylation and transcriptional activity of the glucocorticoid receptor NR3C1 and enhances the interaction of SUMO1 and NR3C1 with UBE2I/UBC9. Has no effect on ubiquitination.

Gene ID: 25950

UniProt: Q9Y3V2