SETD2 antibody (AA 2475-2564)

Catalog No. ABIN7118991

This anti-SETD2 antibody is a Rabbit Polyclonal antibody detecting SETD2 in WB, IHC, ELISA and IF. Suitable for Human, Mouse and Rat.

Quick Overview for SETD2 antibody (AA 2475-2564) (ABIN7118991)

Target: SETD2 (SET Domain Containing 2 (SETD2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SETD2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-SETD2 Antibody

Binding Specificity: AA 2475-2564

Purpose: SETD2 antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen:

Immunogen sequence: 2475-2564aa

Immunogen: SET domain containing 2

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:2000, IHC: 1:20-1:200, IF: 1:10-1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for SETD2

Target: SETD2 (SET Domain Containing 2 (SETD2))

Alternative Name: SETD2

Background:

Synonyms: Histone-lysine N-methyltransferase SETD2|HIF-1|Huntingtin yeast partner B|Huntingtin-interacting protein 1 (HIP-1)|Huntingtin-interacting protein B|Lysine N-methyltransferase 3A|Protein-lysine N-methyltransferase SETD2|SET domain-containing protein 2 (hSET2)|p231HBP|SETD2|HIF1|HYPB|KIAA1732|KMT3A|SET2

Background: Histone methyltransferase that specifically trimethylates 'Lys-36' of histone H3(H3K36me3) using dimethylated 'Lys-36'(H3K36me2) as substrate. Represents the main enzyme generating H3K36me3, a specific tag for epigenetic transcriptional activation. Plays a role in chromatin structure modulation during elongation by coordinating recruitment of the FACT complex and by interacting with hyperphosphorylated POLR2A. Acts as a key regulator of DNA mismatch repair in G1 and early S phase by generating H3K36me3, a mark required to recruit MSH6 subunit of the MutS alpha complex: early recruitment of the MutS alpha complex to chromatin to be replicated allows a quick identification of mismatch DNA to initiate the mismatch repair reaction. H3K36me3 also plays an essential role in the maintenance of a heterochromatic state, by recruiting DNA methyltransferase DNMT3A. H3K36me3 is also enhanced in intron-containing genes, suggesting that SETD2 recruitment is enhanced by splicing and that splicing is coupled to recruitment of elongating RNA polymerase. Required during angiogenesis. Recruited to the promoters of adenovirus 12 E1A gene in case of infection, possibly leading to regulate its expression.

Molecular Weight: 287 kDa

Gene ID: 29072

UniProt: Q9BYW2

Pathways: Tube Formation