SPAG8 antibody

Catalog No. ABIN7119396

This anti-SPAG8 antibody is a Rabbit Polyclonal antibody detecting SPAG8 in ELISA, WB, IHC and IP. Suitable for Human and Mouse.

Quick Overview for SPAG8 antibody (ABIN7119396)

Target: SPAG8 (Sperm Associated Antigen 8 (SPAG8))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPAG8 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Product Details anti-SPAG8 Antibody

Purpose: SPAG8 antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: sperm associated antigen 8

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:2000, IP: 1:200-1:1000, IHC: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for SPAG8

Target: SPAG8 (Sperm Associated Antigen 8 (SPAG8))

Alternative Name: SPAG8

Background:

Synonyms: Sperm-associated antigen 8|HSD-1|Sperm membrane protein 1 (SMP-1)|Sperm membrane protein BS-84|SPAG8

Background: Plays a role in spermatogenesis by enhancing the binding of CREM isoform tau to its coactivator FHL5 and increasing the FHL5-regulated transcriptional activation of CREM isoform tau(By similarity). Involved in the acrosome reaction and in binding of sperm to the zona pellucida(By similarity). Plays a role in regulation of the cell cycle by controlling progression through the G2/M phase, possibly by delaying the activation of CDK1 which is required for entry into mitosis(PubMed:19548270). May play a role in fertility and microtubule formation through interaction with RANBP9(PubMed:10500252).

Molecular Weight: 56-65 kDa

Gene ID: 26206

UniProt: Q99932