SUV39H1 antibody

Catalog No. ABIN7119616

This Rabbit Polyclonal antibody specifically detects SUV39H1 in WB, ELISA and IP. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for SUV39H1 antibody (ABIN7119616)

Target: SUV39H1 (Suppressor of Variegation 3-9 Homolog 1 (Drosophila) (SUV39H1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SUV39H1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Product Details anti-SUV39H1 Antibody

Purpose: SUV39H1 antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: suppressor of variegation 3-9 homolog 1(Drosophila)

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:2000, IP: 1:200-1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for SUV39H1

Target: SUV39H1 (Suppressor of Variegation 3-9 Homolog 1 (Drosophila) (SUV39H1))

Alternative Name: SUV39H1

Background:

Synonyms: Histone-lysine N-methyltransferase SUV39H1|Histone H3-K9 methyltransferase 1 (H3-K9-HMTase 1)|Lysine N-methyltransferase 1A|Position-effect variegation 3-9 homolog|Suppressor of variegation 3-9 homolog 1 (Su(var)3-9 homolog 1)|SUV39H1|KMT1A|SUV39H

Background: Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3 using monomethylated H3 'Lys-9' as substrate. Also weakly methylates histone H1(in vitro). H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1(CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin at pericentric and telomere regions. H3 'Lys-9' trimethylation is also required to direct DNA methylation at pericentric repeats. SUV39H1 is targeted to histone H3 via its interaction with RB1 and is involved in many processes, such as repression of MYOD1-stimulated differentiation, regulation of the control switch for exiting the cell cycle and entering differentiation, repression by the PML-RARA fusion protein, BMP-induced repression, repression of switch recombination to IgA and regulation of telomere length. Component of the eNoSC(energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD(+)/NADP(+) ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys-9'(H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus. Recruited by the large PER complex to the E-box elements of the circadian target genes such as PER2 itself or PER1, contributes to the conversion of local chromatin to a heterochromatin-like repressive state through H3 'Lys-9' trimethylation.

Molecular Weight: 48-50 kDa

Gene ID: 6839

UniProt: O43463