TSH receptor antibody

Catalog No. ABIN7119962

The Rabbit Polyclonal anti-TSH receptor antibody has been validated for WB, ELISA, IHC and IF. It is suitable to detect TSH receptor in samples from Human, Mouse and Rat.

Quick Overview for TSH receptor antibody (ABIN7119962)

Target: TSH receptor (TSHR) (Thyroid Stimulating Hormone Receptor (TSHR))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TSH receptor antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-TSH receptor Antibody

Purpose: TSHR antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen:

Immunogen sequence: P16473-2,21-253AA

Immunogen: thyroid stimulating hormone receptor

Isotype: IgG

Application Details

Application Notes: WB: 1:500 - 1:2000, IHC: 1:50 - 1:200, IF: 1:20- 1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for TSH receptor

Target: TSH receptor (TSHR) (Thyroid Stimulating Hormone Receptor (TSHR))

Alternative Name: TSHR

Background:

Synonyms: Thyrotropin receptor|Thyroid-stimulating hormone receptor (TSH-R)|TSHR|LGR3

Background: The protein encoded by this gene is a membrane protein and a major controller of thyroid cell metabolism. The encoded protein is a receptor for thyrothropin and thyrostimulin, and its activity is mediated by adenylate cyclase. Defects in this gene are a cause of several types of hyperthyroidism. The gene of TSHR maps to chromosome 14q31.1, and encodes a 764-amino acid protein with a predicted unmodified molecular weight of 86.8 kDa. The apparent molecular weight of glycosylated form is 95-120 kDa. Studies reveal that TSHR is composed of two subunits, A and B, which are produced by cleavage of single-chain TSHR on the cell surface and subsequently connected by disulfide bonds. This rabbit polyclonal antibody can detect glycosylated subunit A with an experimentally determined molecular weight of 62-70 kDa under reducing condition. (PMID: 20578897, 17158770, 12593718)

Molecular Weight: 62-100 kDa

Gene ID: 7253

UniProt: P16473

Pathways: Thyroid Hormone Synthesis