TNIP1 antibody

Catalog No. ABIN7119995

The Rabbit Polyclonal anti-TNIP1 antibody has been validated for WB, ELISA, IHC, IF and IP. It is suitable to detect TNIP1 in samples from Human.

Quick Overview for TNIP1 antibody (ABIN7119995)

Target: TNIP1 (TNFAIP3 Interacting Protein 1 (TNIP1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TNIP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Product Details anti-TNIP1 Antibody

Purpose: TNIP1 antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: TNFAIP3 interacting protein 1

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:2000, IP: 1:200-1:1000, IHC: 1:20-1:200, IF: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for TNIP1

Target: TNIP1 (TNFAIP3 Interacting Protein 1 (TNIP1))

Alternative Name: TNIP1

Background:

Synonyms: TNFAIP3-interacting protein 1|A20-binding inhibitor of NF-kappa-B activation 1 (ABIN-1)|HIV-1 Nef-interacting protein|Nef-associated factor 1 (Naf1)|Nip40-1|Virion-associated nuclear shuttling protein (VAN, hVAN)|TNIP1|KIAA0113|NAF1

Background: Inhibits NF-kappa-B activation and TNF-induced NF-kappa-B-dependent gene expression by regulating A20/TNFAIP3-mediated deubiquitination of IKBKG, proposed to link A20/TNFAIP3 to ubiquitinated IKBKG. Involved in regulation of EGF-induced ERK1/ERK2 signaling pathway, blocks MAPK3/MAPK1 nuclear translocation and MAPK1-dependent transcription. Increases cell surface CD4(T4) antigen expression. Involved in the anti-inflammatory response of macrophages and positively regulates TLR-induced activation of CEBPB. Involved in the prevention of autoimmunity, this function implicates binding to polyubiquitin. Involved in leukocyte integrin activation during inflammation, this function is mediated by association with SELPLG and dependent on phosphorylation by SRC-family kinases. Interacts with HIV-1 matrix protein and is packaged into virions and overexpression can inhibit viral replication. May regulate matrix nuclear localization, both nuclear import of PIC(Preintegration complex) and export of GAG polyprotein and viral genomic RNA during virion production. In case of infection, promotes association of IKBKG with Shigella flexneri E3 ubiquitin-protein ligase ipah9.8 p which in turn promotes polyubiquitination of IKBKG leading to its proteasome-dependent degradation and thus is perturbing NF-kappa-B activation during bacterial infection.

Molecular Weight: 72 kDa

Gene ID: 10318

UniProt: Q15025

Pathways: Activation of Innate immune Response