USP13 antibody

Catalog No. ABIN7120497

This anti-USP13 antibody is a Mouse Monoclonal antibody detecting USP13 in WB, IHC, ELISA and IF. Suitable for Human.

Quick Overview for USP13 antibody (ABIN7120497)

Target: USP13 (Ubiquitin Specific Peptidase 13 (Isopeptidase T-3) (USP13))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This USP13 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Clone: 9E6

Product Details anti-USP13 Antibody

Purification: Protein A+G purification

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: ubiquitin specific peptidase 13(isopeptidase T-3)

Isotype: IgG1

Application Details

Application Notes: WB: 1:500-1:5000, IHC: 1:20-1:200, IF: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

Expiry Date: 12 months

Target Details for USP13

Target: USP13 (Ubiquitin Specific Peptidase 13 (Isopeptidase T-3) (USP13))

Alternative Name: USP13

Background: Synonyms:Deubiquitinating enzyme 13, Isopeptidase T 3, IsoT 3, ISOT3, Ubiquitin thiolesterase 13, USP13 Background:Deubiquitinase that mediates deubiquitination of target proteins such as BECN1, MITF, SKP2 and USP10 and is involved in various processes such as autophagy and endoplasmic reticulum-associated degradation(ERAD). Component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34-containing complexes. Also deubiquitinates USP10, an essential regulator of p53/TP53 stability. In turn, PIK3C3/VPS34-containing complexes regulate USP13 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13. Recruited by nuclear UFD1 and mediates deubiquitination of SKP2, thereby regulating endoplasmic reticulum-associated degradation(ERAD). Mediates stabilization of SIAH2 independently of deubiquitinase activity: binds ubiquitinated SIAH2 and acts by impairing SIAH2 autoubiquitination. Has a weak deubiquitinase activity in vitro and preferentially cleaves 'Lys-63'-linked polyubiquitin chains. In contrast to USP5, it is not able to mediate unanchored polyubiquitin disassembly. Able to cleave ISG15 in vitro, however, additional experiments are required to confirm such data.

Molecular Weight: 97 kDa

Gene ID: 8975

UniProt: Q92995

Pathways: SARS-CoV-2 Protein Interactome