USP14 antibody

Catalog No. ABIN7120498

This Rabbit Polyclonal antibody specifically detects USP14 in WB, ELISA, IF and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for USP14 antibody (ABIN7120498)

Target: USP14 (Ubiquitin Specific Peptidase 14 (USP14))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This USP14 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-USP14 Antibody

Purpose: USP14 antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: ubiquitin specific peptidase 14(tRNA-guanine transglycosylase)

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:5000, IP: 1:500-1:5000, IF: 1:10-1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for USP14

Target: USP14 (Ubiquitin Specific Peptidase 14 (USP14))

Alternative Name: USP14

Background:

Synonyms: Ubiquitin carboxyl-terminal hydrolase 14|Deubiquitinating enzyme 14|Ubiquitin thioesterase 14|Ubiquitin-specific-processing protease 14|USP14|TGT

Background: Proteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated proteins. Ensures the regeneration of ubiquitin at the proteasome. Is a reversibly associated subunit of the proteasome and a large fraction of proteasome-free protein exists within the cell. Required for the degradation of the chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis. Serves also as a physiological inhibitor of endoplasmic reticulum-associated degradation(ERAD) under the non-stressed condition by inhibiting the degradation of unfolded endoplasmic reticulum proteins via interaction with ERN1. Indispensable for synaptic development and function at neuromuscular junctions(NMJs).

Molecular Weight: 52-60 kDa

Gene ID: 9097

UniProt: P54578