USP15 antibody

Catalog No. ABIN7120499

The Rabbit Polyclonal anti-USP15 antibody has been validated for WB, ELISA and IP. It is suitable to detect USP15 in samples from Human, Mouse and Rat.

Quick Overview for USP15 antibody (ABIN7120499)

Target: USP15 (Ubiquitin Specific Peptidase 15 (USP15))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This USP15 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Product Details anti-USP15 Antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: ubiquitin specific peptidase 15

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:5000, IP: 1:500-1:5000, IHC: 1:50-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

Expiry Date: 12 months

Target Details for USP15

Target: USP15 (Ubiquitin Specific Peptidase 15 (USP15))

Alternative Name: USP15

Background: Synonyms:Deubiquitinating enzyme 15, KIAA0529, Ubiquitin thiolesterase 15, Unph 2, UNPH4, USP15 Background:Hydrolase that removes conjugated ubiquitin from target proteins and regulates various pathways such as the TGF-beta receptor signaling and NF-kappa-B pathways. Acts as a key regulator of TGF-beta receptor signaling pathway, but the precise mechanism is still unclear: according to a report, acts by promoting deubiquitination of monoubiquitinated R-SMADs(SMAD1, SMAD2 and/or SMAD3), thereby alleviating inhibition of R-SMADs and promoting activation of TGF-beta target genes(PubMed:21947082). According to another reports, regulates the TGF-beta receptor signaling pathway by mediating deubiquitination and stabilization of TGFBR1, leading to an enhanced TGF-beta signal(PubMed:22344298). Able to mediate deubiquitination of monoubiquitinated substrates as well as 'Lys-48'-linked polyubiquitin chains, protecting them against proteasomal degradation. May also regulate gene expression and/or DNA repair through the deubiquitination of histone H2B(PubMed:24526689). Acts as an associated component of COP9 signalosome complex(CSN) and regulates different pathways via this association: regulates NF-kappa-B by mediating deubiquitination of NFKBIA and deubiquitinates substrates bound to VCP. Protects APC and human papillomavirus type 16 protein E6 against degradation via the ubiquitin proteasome pathway.

Molecular Weight: 112 kDa

Gene ID: 9958

UniProt: Q9Y4E8