USP5 antibody (AA 4-165)

Catalog No. ABIN7120527

This anti-USP5 antibody is a Rabbit Polyclonal antibody detecting USP5 in WB, ELISA and IP. Suitable for Human, Mouse and Rat.

Quick Overview for USP5 antibody (AA 4-165) (ABIN7120527)

Target: USP5 (Ubiquitin Specific Peptidase 5 (USP5))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This USP5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Product Details anti-USP5 Antibody

Binding Specificity: AA 4-165

Purpose: USP5 antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen:

Immunogen sequence: 4-165aa

Immunogen: ubiquitin specific peptidase 5(isopeptidase T)

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:5000, IP: 1:500-1:5000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for USP5

Target: USP5 (Ubiquitin Specific Peptidase 5 (USP5))

Alternative Name: USP5

Background:

Synonyms: Ubiquitin carboxyl-terminal hydrolase 5|Deubiquitinating enzyme 5|Isopeptidase T|Ubiquitin thioesterase 5|Ubiquitin-specific-processing protease 5|USP5|ISOT

Background: Cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. Involved in unanchored 'Lys-48'-linked polyubiquitin disassembly. Binds linear and 'Lys-63'-linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition.

Molecular Weight: 93 kDa

Gene ID: 8078

UniProt: P45974