USP5 antibody

Catalog No. ABIN7120529

The Mouse Monoclonal anti-USP5 antibody has been validated for WB and ELISA. It is suitable to detect USP5 in samples from Human.

Quick Overview for USP5 antibody (ABIN7120529)

Target: USP5 (Ubiquitin Specific Peptidase 5 (USP5))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This USP5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Clone: 6G1

Product Details anti-USP5 Antibody

Purification: Protein A+G purification

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: ubiquitin specific peptidase 5(isopeptidase T)

Isotype: IgG1

Application Details

Application Notes: WB: 1:500-1:5000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

Expiry Date: 12 months

Target Details for USP5

Target: USP5 (Ubiquitin Specific Peptidase 5 (USP5))

Alternative Name: USP5

Background: Synonyms:Deubiquitinating enzyme 5, Isopeptidase T, ISOT, Ubiquitin thiolesterase 5, USP5 Background:Cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. Involved in unanchored 'Lys-48'-linked polyubiquitin disassembly. Binds linear and 'Lys-63'-linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition.

Molecular Weight: 93 kDa

Gene ID: 8078

UniProt: P45974