UBE2C antibody

Catalog No. ABIN7120541

The Mouse Monoclonal anti-UBE2C antibody is suitable to detect UBE2C in samples from Human. It has been validated for WB, ELISA, IHC and IF.

Quick Overview for UBE2C antibody (ABIN7120541)

Target: UBE2C (Ubiquitin-Conjugating Enzyme E2C (UBE2C))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This UBE2C antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: 7E3

Product Details anti-UBE2C Antibody

Purpose: UBE2C antibody

Purification: Protein A+G purification

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: ubiquitin-conjugating enzyme E2C

Isotype: IgG1

Application Details

Application Notes: WB: 1:500-1:2000, IHC: 1:20-1:200, IF: 1:10-1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for UBE2C

Target: UBE2C (Ubiquitin-Conjugating Enzyme E2C (UBE2C))

Alternative Name: UBE2C

Background:

Synonyms: Ubiquitin-conjugating enzyme E2 C|(E3-independent) E2 ubiquitin-conjugating enzyme C|E2 ubiquitin-conjugating enzyme C|UbcH10|Ubiquitin carrier protein C|Ubiquitin-protein ligase C|UBE2C|UBCH10

Background: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'-and 'Lys-48'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome(APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by initiating 'Lys-11'-linked polyubiquitin chains on APC/C substrates, leading to the degradation of APC/C substrates by the proteasome and promoting mitotic exit.

Molecular Weight: 17 kDa

Gene ID: 11065

UniProt: O00762

Pathways: Ubiquitin Proteasome Pathway