UBE2F antibody

Catalog No. ABIN7120548

The Rabbit Polyclonal anti-UBE2F antibody (ABIN7120548) specifically detects UBE2F in WB, ELISA, IHC and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for UBE2F antibody (ABIN7120548)

Target: UBE2F (Ubiquitin-Conjugating Enzyme E2F (UBE2F))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE2F antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-UBE2F Antibody

Purpose: UBE2F antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: ubiquitin-conjugating enzyme E2F(putative)

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:2000, IHC: 1:20-1:200, IF: 1:50-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for UBE2F

Target: UBE2F (Ubiquitin-Conjugating Enzyme E2F (UBE2F))

Alternative Name: UBE2F

Background:

Synonyms: NEDD8-conjugating enzyme UBE2F|NEDD8 carrier protein UBE2F|NEDD8 protein ligase UBE2F|NEDD8-conjugating enzyme 2|RING-type E3 NEDD8 transferase UBE2F|Ubiquitin-conjugating enzyme E2 F|UBE2F|NCE2

Background: Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5.

Molecular Weight: 21 kDa

Gene ID: 140739

UniProt: Q969M7