Ube2t antibody

Catalog No. ABIN7120563

This anti-Ube2t antibody is a Rabbit Polyclonal antibody detecting Ube2t in WB, ELISA and IP. Suitable for Human, Mouse and Rat.

Quick Overview for Ube2t antibody (ABIN7120563)

Target: Ube2t (Ubiquitin-Conjugating Enzyme E2T (Ube2t))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ube2t antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Product Details anti-Ube2t Antibody

Purpose: UBE2T antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: ubiquitin-conjugating enzyme E2T(putative)

Isotype: IgG

Application Details

Application Notes: WB: 1:200-1:2000, IP: 1:200-1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for Ube2t

Target: Ube2t (Ubiquitin-Conjugating Enzyme E2T (Ube2t))

Alternative Name: UBE2T

Background:

Synonyms: Ubiquitin-conjugating enzyme E2 T|Cell proliferation-inducing gene 50 protein|E2 ubiquitin-conjugating enzyme T|Ubiquitin carrier protein T|Ubiquitin-protein ligase T|UBE2T

Background: The ubiquitin(Ub)-mediated protein degradation pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub to specific protein substrates. The first step requires ATP-dependent activation of the C-terminus of Ub and the assembly of multi-Ubs by Ub-activating enzyme E1. The ubiquitin-conjugating enzyme E2, catalytic(UBCc) domain, is then conjugated to Ubs, through a thiol-ester linkage between a conserved cysteine and the C-terminus of Ub, to generate an intermediate Ub-E2 complex. Then the E3, a ligase, catalyzes the transfer of Ub from E2 to the appropriate substrate. This pathway regulates many fundamental cellular processes. There are also other E2s which form thiol-ester linkages without the use of E3s as well as several UBC homologs(TSG101, Mms2, Croc-1 and similar proteins), which lack the active site cysteine essential for ubiquitination and appear to function in DNA repair pathways.

Molecular Weight: 24 kDa

Gene ID: 28089

UniProt: Q9NPD8

Pathways: Chromatin Binding