UPF3A antibody

Catalog No. ABIN7120628

The Rabbit Polyclonal anti-UPF3A antibody has been validated for WB, ELISA and IHC. It is suitable to detect UPF3A in samples from Human, Mouse and Rat.

Quick Overview for UPF3A antibody (ABIN7120628)

Target: UPF3A (UPF3 Regulator of Nonsense Transcripts Homolog A (UPF3A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UPF3A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-UPF3A Antibody

Purpose: UPF3A antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: UPF3 regulator of nonsense transcripts homolog A(yeast)

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:5000, IHC:1:20-1:200,IF: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for UPF3A

Target: UPF3A (UPF3 Regulator of Nonsense Transcripts Homolog A (UPF3A))

Alternative Name: UPF3A

Background:

Synonyms: Regulator of nonsense transcripts 3A|Nonsense mRNA reducing factor 3A|Up-frameshift suppressor 3 homolog A (hUpf3)|UPF3A|RENT3A|UPF3

Background: Involved in nonsense-mediated decay(NMD) of mRNAs containing premature stop codons by associating with the nuclear exon junction complex(EJC) and serving as link between the EJC core and NMD machinery. Recruits UPF2 at the cytoplasmic side of the nuclear envelope and the subsequent formation of an UPF1-UPF2-UPF3 surveillance complex(including UPF1 bound to release factors at the stalled ribosome) is believed to activate NMD. However, UPF3A is shown to be only marginally active in NMD as compared to UPF3B. Binds spliced mRNA upstream of exon-exon junctions. In vitro, weakly stimulates translation.

Molecular Weight: 51 kDa

Gene ID: 65110

UniProt: Q9H1J1