WWP1 antibody

Catalog No. ABIN7120866

This anti-WWP1 antibody is a Rabbit Polyclonal antibody detecting WWP1 in WB, ELISA, IP, IHC and IF. Suitable for Human, Mouse and Rat.

Quick Overview for WWP1 antibody (ABIN7120866)

Target: WWP1 (WW Domain Containing E3 Ubiquitin Protein Ligase 1 (WWP1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WWP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-WWP1 Antibody

Purpose: WWP1 antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: WW domain containing E3 ubiquitin protein ligase 1

Isotype: IgG

Application Details

Application Notes: WB: 1:200-1:2000, IP: 1:200-1:1000, IF: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for WWP1

Target: WWP1 (WW Domain Containing E3 Ubiquitin Protein Ligase 1 (WWP1))

Alternative Name: WWP1

Background:

Synonyms: NEDD4-like E3 ubiquitin-protein ligase WWP1|Atrophin-1-interacting protein 5 (AIP5)|HECT-type E3 ubiquitin transferase WWP1|TGIF-interacting ubiquitin ligase 1 (Tiul1)|WW domain-containing protein 1|WWP1

Background: E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Ubiquitinates ERBB4 isoforms JM-A CYT-1 and JM-B CYT-1, KLF2, KLF5 and TP63 and promotes their proteasomal degradation. Ubiquitinates RNF11 without targeting it for degradation. Ubiquitinates and promotes degradation of TGFBR1, the ubiquitination is enhanced by SMAD7. Ubiquitinates SMAD6 and SMAD7. Ubiquitinates and promotes degradation of SMAD2 in response to TGF-beta signaling, which requires interaction with TGIF.

Molecular Weight: 90-110 kDa

Gene ID: 11059

UniProt: Q9H0M0