YTHDC1 antibody

Catalog No. ABIN7120901

The Rabbit Polyclonal anti-YTHDC1 antibody is suitable to detect YTHDC1 in samples from Human, Mouse and Rat. It has been validated for WB and ELISA.

Quick Overview for YTHDC1 antibody (ABIN7120901)

Target: YTHDC1 (YTH Domain Containing 1 (YTHDC1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This YTHDC1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-YTHDC1 Antibody

Purpose: YTHDC1 antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: YTH domain containing 1

Isotype: IgG

Application Details

Application Notes: WB: 1:200-1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for YTHDC1

Target: YTHDC1 (YTH Domain Containing 1 (YTHDC1))

Alternative Name: YTHDC1

Background:

Synonyms: YTH domain-containing protein 1|Splicing factor YT521 (YT521-B)|YTHDC1|KIAA1966|YT521

Background: Regulator of alternative splicing that specifically recognizes and binds N6-methyladenosine(m6A)-containing RNAs(PubMed:26318451, PubMed:26876937, PubMed:25242552). M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability(PubMed:26318451, PubMed:25242552). Acts as a key regulator of exon-inclusion or exon-skipping during alternative splicing via interaction with mRNA splicing factors SRSF3 and SRSF10(PubMed:26876937). Specifically binds m6A-containing mRNAs and promotes recruitment of SRSF3 to its mRNA-binding elements adjacent to m6A sites, leading to exon-inclusion during alternative splicing(PubMed:26876937). In contrast, interaction with SRSF3 prevents interaction with SRSF10, a splicing factor that promotes exon skipping: this prevents SRSF10 from binding to its mRNA-binding sites close to m6A-containing regions, leading to inhibit exon skipping during alternative splicing(PubMed:26876937). May also regulate alternative splice site selection(PubMed:20167602).

Molecular Weight: 110 kDa

Gene ID: 91746

UniProt: Q96MU7