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Tissue factor antibody (Coagulation Factor III (thromboplastin, Tissue Factor)) AA 32-100 Primary Antibody

F3 Reactivity: Human, Mouse, Rat, Pig ELISA, FACS, IF (cc), IF (p), IHC (fro), IHC (p), WB Host: Rabbit Polyclonal unconjugated
Pubmed (7 references) Independent Validation (1 validation)
Catalog No. ABIN708086
$374.00
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100 μL
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  • Target See all Tissue factor (F3) Antibodies
    Tissue factor (F3)
    Binding Specificity
    • 15
    • 9
    • 8
    • 8
    • 7
    • 6
    • 4
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 32-100
    Reactivity
    • 97
    • 40
    • 34
    • 15
    • 12
    • 3
    • 2
    • 1
    Human, Mouse, Rat, Pig
    Host
    • 85
    • 39
    • 7
    • 4
    Rabbit
    Clonality
    • 85
    • 49
    Polyclonal
    Conjugate
    • 63
    • 22
    • 14
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Tissue factor antibody is un-conjugated
    Application
    • 83
    • 40
    • 39
    • 38
    • 19
    • 16
    • 13
    • 12
    • 12
    • 8
    • 6
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    ELISA, Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
    Predicted Reactivity
    Dog,Cow,Horse,Rabbit,Guinea Pig
    Purification
    Purified by Protein A.
    Immunogen
    KLH conjugated synthetic peptide derived from human CD142
    Isotype
    IgG
  • Application Notes
    WB 1:300-5000
    ELISA 1:500-1000
    FCM 1:20-100
    IHC-P 1:200-400
    IHC-F 1:100-500
    IF(IHC-P) 1:50-200
    IF(IHC-F) 1:50-200
    IF(ICC) 1:50-200
    Restrictions
    For Research Use only
  • Validation #029589 (Immunohistochemistry)
    'Independent Validation' Badge
    by
    Reveal Biosciences
    No.
    #029589
    Date
    02/07/2014
    Antigen
    Lot Number
    980886W
    Method validated
    Immunohistochemistry
    Positive Control
    Mouse lung tissue
    Negative Control
    Mouse brain tissue
    Notes
    Coagulation Factor III (thromboplastin, Tissue Factor) (F3) staining is observed in positive control tissue and not in negative control tissue.
    'Independent Validation' Badge
    Validation Images
    Full Methods
    Primary Antibody
    • Antigen: Coagulation Factor III (thromboplastin, Tissue Factor) (F3)
    • Catalog number: ABIN708086
    • Lot number: 120224
    Secondary Antibody
    • Antibody: Bond Polymer Refine Detection Kit
    • Lot number: 24144
    Full Protocol
    • Immunohistochemistry was performed on a Leica Bond automated immunostainer.
    • Sections were deparaffinized with Novocastra Bond Dewax Solution and rehydrated into Leica Bond Wash Buffer.
    • Sections were heated to 98 °C for 20 minutes in 10 mM citrate buffer pH 9.0 (ER1; Leica) for antigen retrieval.
    • Sections were blocked in 3 % casein plus 0.1 % Triton-X100 for 10 minutes at room temperature.
    • Sections were washed x 3 in Leica Bond Wash Buffer.
    • Sections were incubated with primary antibody diluted 1:200 in Universal Antibody Dilution Buffer (Electron Microscopy Sciences, 25886-05) for 15 minutes at room temperature.
    • Sections were washed x 3 in Leica Bond Wash Buffer.
    • Sections were incubated with Leica Bond Polymer for 8 minutes at room temperature.
    • Sections were washed x 4 in Leica Bond Wash Buffer.
    • Sections were washed x 1 in Distilled Water.
    • Sections were incubated with Peroxide Block (Leica) for 10 min to block endogenous peroxidase.
    • Sections were washed x 4 in Leica Bond Wash Buffer.
    • Sections were incubated with DAB chromogenic substrate (Leica) for 10 min at RT.
    • Sections were washed x 3 in Distilled Water.
    • Sections were counterstained with hematoxylin (Leica) for 2 minutes.
    • Sections were washed x 1 in Distilled Water.
    • Sections were washed x 1 in Leica Bond Wash Buffer.
    • Sections were washed x 1 in Distilled Water.
    • Sections were dehydrated, mounted and photographed under a light microscope.
    Experimental Notes
    None notes.
  • Format
    Liquid
    Concentration
    1 μg/μL
    Buffer
    0.01M TBS( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.
    Precaution of Use
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
    Expiry Date
    12 months
  • Fletcher, Das, Gadye, Street, Baudhuin, Wagner, Cole, Flores, Choi, Yosef, Purdom, Dudoit, Risso, Ngai: "Deconstructing Olfactory Stem Cell Trajectories at Single-Cell Resolution." in: Cell stem cell, Vol. 20, Issue 6, pp. 817-830.e8, (2017) (PubMed).

    Kim, Yoo, Gu, Kim: "Histones Induce the Procoagulant Phenotype of Endothelial Cells through Tissue Factor Up-Regulation and Thrombomodulin Down-Regulation." in: PLoS ONE, Vol. 11, Issue 6, pp. e0156763, (2016) (PubMed).

    Yoo, Kim, Gu, Lee, Lee, Hwang, Hwang, Kim, Kim: "Porcine endothelium induces DNA-histone complex formation in human whole blood: a harmful effect of histone on coagulation and endothelial activation." in: Xenotransplantation, Vol. 23, Issue 6, pp. 464-471, (2016) (PubMed).

    Gleeson, Martin, Ali, Kumar, Pillai, Kumar, OSullivan, Whelan, Stocca, Khider, Barry, OBrien, Caplice: "Bone Marrow-Derived Mesenchymal Stem Cells Have Innate Procoagulant Activity and Cause Microvascular Obstruction Following Intracoronary Delivery: Amelioration by Antithrombin Therapy." in: Stem cells (Dayton, Ohio), (2015) (PubMed).

    Tian, Salsbery, Wang, Yuan, Yang, Zhao, Wu, Zhang, Konkle, Thiagarajan, Li, Zhang, Dong: "Brain-derived microparticles induce systemic coagulation in a murine model of traumatic brain injury." in: Blood, (2015) (PubMed).

    Milano, Dongiovanni, Artoni, Gatti, Rosso, Colombo, Bollati, Maggioni, Mannucci, Bertazzi, Fargion, Valenti: "Particulate matter phagocytosis induces tissue factor in differentiating macrophages." in: Journal of applied toxicology : JAT, (2015) (PubMed).

    Liu, Wei, Zhang, Xu, Wang, Zhou: "Procoagulant activity and cellular origin of microparticles in human amniotic fluid." in: Thrombosis research, Vol. 133, Issue 4, pp. 645-51, (2014) (PubMed).

  • Target
    Tissue factor (F3)
    Alternative Name
    CD142 (F3 Products)
    Synonyms
    CD142, TF, TFA, PRO1557, PRO2086, TFQTL1, f3, AA409063, Cf-3, Cf3, tf, zgc:112151, coagulation factor III, tissue factor, transferrin, coagulation factor IIIa, coagulation factor III, tissue factor, coagulation factor IIIb, coagulation factor III (thromboplastin, tissue factor) S homeolog, F3, TF, f3a, tf, f3b, f3.S
    Background

    Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.

    Subcellular location: Secreted, Cell membrane

    Synonyms: TF, TFA, CD142, Tissue factor, Coagulation factor III, Thromboplastin, F3

    Gene ID
    2152
    UniProt
    P13726
    Pathways
    Positive Regulation of Endopeptidase Activity, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling
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