SMNDC1 antibody

Catalog No. ABIN7504151

This Mouse Monoclonal antibody specifically detects SMNDC1 in FACS and IF. It exhibits reactivity toward Human.

Quick Overview for SMNDC1 antibody (ABIN7504151)

Target: SMNDC1 (Survival Motor Neuron Domain Containing 1 (SMNDC1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SMNDC1 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Product Details anti-SMNDC1 Antibody

Specificity: SPF30 (survival of motor neuron-related-splicing factor 30), also known as SMNDC1 (survival motor neuron domain containing 1) or SMNR (SMN-related protein), is an essential splicing factor required for spliceosome assembly that belongs to the SMN family. It contains one Tudor domain with significant similarity to SMN (survival motor neuron) and is expressed in skeletal muscle, pancreas and heart, localizing to Cajal bodies and nuclear speckles. SPF30 plays an important role in spliceosome assembly and directly interacts with five U snRNPs. The loss of SPF30 causes spliceosome assembly to arrest at prespliceosomes (A complex). This supports a function for SPF30 in mediating the incorporation/recruitment of U4/U5/U6 tri-snRNP to the prespliceosome. In addition, the overexpression of SPF30 can lead to apoptosis.

Cross-Reactivity (Details): Human.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human SMNDC1 protein

Isotype: IgG2b

Application Details

Application Notes:

Positive Control: HeLa or HepG2 cells.

Known Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA and 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody is stable for 24 months. Non-hazardous. Also available WITHOUT BSA & azide at 1.0mg/ml.

Expiry Date: 24 months

Target Details for SMNDC1

Target: SMNDC1 (Survival Motor Neuron Domain Containing 1 (SMNDC1))

Alternative Name: SMNDC1

Background: 30 kDa splicing factor SMNrp, MGC106917, MGC112663, SMN related protein, SMNR, SPF30, Splicing factor 30, survival of motor neuron-related, Survival motor neuron domain containing protein 1, Survival of motor neuron related splicing factor 30,SMNDC1
Cellular localisation: Nucleus. Detected in nuclear speckles containing snRNP and in Cajal (coiled) bodies.

Molecular Weight: 30kDa

Gene ID: 10285, 632093

UniProt: O75940