TDRKH antibody

Catalog No. ABIN7504177

The Mouse Monoclonal anti-TDRKH antibody has been validated for WB, IF, IP, FACS and IHC (f). It is suitable to detect TDRKH in samples from Human.

Quick Overview for TDRKH antibody (ABIN7504177)

Target: TDRKH (Tudor and KH Domain Containing (TDRKH))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TDRKH antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Product Details anti-TDRKH Antibody

Specificity: Participates in the primary piRNA biogenesis pathway and is required during spermatogenesis to repress transposable elements and prevent their mobilization, which is essential for the germline integrity. The piRNA metabolic process mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Required for the final steps of primary piRNA biogenesis by participating in the processing of 31-37 nt intermediates into mature piRNAs. May act in pi-bodies and piP-bodies by transferring piRNA precursors or intermediates to or between these granules.

Cross-Reactivity (Details): Human. Predicted to react with Mouse and Rat.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human TDRKH protein

Isotype: IgG2b

Application Details

Application Notes:

Positive Control: HeLa or MCF7 cells. Human testis or brain.

Known Application: Immunoprecipitation (1-2 μg per 100-500 μg of total protein), Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA and 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody is stable for 24 months. Non-hazardous. Also available WITHOUT BSA & azide at 1.0mg/ml.

Expiry Date: 24 months

Target Details for TDRKH

Target: TDRKH (Tudor and KH Domain Containing (TDRKH))

Alternative Name: TDRKH

Background: 2700091C21Rik, Putative RNA binding protein, RGD1311196, RP11-98D18.8, TDRD2, TDRD7, Tdrkh, Tudor and KH domain containing, Tudor and KH domain-containing protein, tudor domain containing 2,TDRKH
Cellular localisation: Cytoplasm. Localized to cytosol and centrosome.

Molecular Weight: 62.05kDa

Gene ID: 11022, 144439

UniProt: Q9Y2W6