Follistatin antibody
Quick Overview for Follistatin antibody (ABIN7126189)
Target
See all Follistatin (FST) AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Specificity
- Follistatin is a single-chain glycosylated protein that inhibits follicle stimulating hormone (FSH) release. Alternative splicing of Follistatin mRNA yields two isoforms, FS315 and FS288. FS288 is the main cell-surface form and binds to surface heparin sulphate proteoglycans. FST/4281 is a clone raised against recombinant Fst 288, and is used in combination, commonly as the capture, with antibody FST/4282 in a two site ELISA for the detection of Follistatin.
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Cross-Reactivity (Details)
- Human.
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Purification
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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Immunogen
- Raised to human Follistatin (Fst) 288 and recognizes both human Fst 288 and 315
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Isotype
- IgG1
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Application Notes
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Known_Application: ELISA (Use in combination with clone FST/4282 for two-site ELISA), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Positive_Control: Human testis or ovary.
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Restrictions
- For Research Use only
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Concentration
- 1.0 mg/mL
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Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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Preservative
- Azide free
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Storage
- -20 °C,-80 °C
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Storage Comment
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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Expiry Date
- 24 months
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- Follistatin (FST)
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Alternative Name
- FST
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Background
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Follistatin, FS, FST, Active-binding protein,Follistatin / Activin Binding Protein
Cellular localisation: Secreted. -
Molecular Weight
- 37kDa
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Gene ID
- 10468, 9914
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UniProt
- P19883
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Pathways
- Negative Regulation of Hormone Secretion
Target
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