UBE2C antibody

Catalog No. ABIN7126211

This Mouse Monoclonal antibody specifically detects UBE2C in ELISA, Coat and IHC (f). It exhibits reactivity toward Human.

Quick Overview for UBE2C antibody (ABIN7126211)

Target: UBE2C (Ubiquitin-Conjugating Enzyme E2C (UBE2C))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This UBE2C antibody is un-conjugated

Application: ELISA, Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Product Details anti-UBE2C Antibody

Specificity: The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. The first step requires the ATP-dependent activation of the Ub C-terminus and the assembly of multi-Ub chains by the Ub-activating enzyme known as the E1 component. The Ub chain is then conjugated to the Ubconjugating enzyme (E2) to generate an intermediate Ub-E2 complex. The Ub-ligase (E3) then catalyzes the transfer of Ub from E2 to the appropriate protein substrate. UBE2C, also designated UBCH10 in human, is an E2 ubiquitin conjugating enzyme for the anaphase promoting complex (APC), which coordinates mitosis and G1 by sequentially promoting the degradation of key cell-cycle regulators. UBE2C is overexpressed in many different types of cancers and may be a potential therapeutic target.

Cross-Reactivity (Details): Human

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human full-length UBE2C protein

Isotype: IgG2b

Application Details

Application Notes:

Known_Application: ELISA (For coating, order antibody without BSA),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: HeLa, Jurkat or A431 cells. Human tonsil or placenta tissue.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for UBE2C

Target: UBE2C (Ubiquitin-Conjugating Enzyme E2C (UBE2C))

Alternative Name: UBE2C

Background: Cyclin selective ubiquitin carrier protein, mitotic specific ubiquitin conjugating enzyme, UB E2C, UBCH10, Ubiquitin protein ligase C,Ubiquitin-conjugating Enzyme E2C / UBCH10
Cellular localisation: Cytoplasm.

Molecular Weight: 20kDa

Gene ID: 11065, 93002

UniProt: O00762

Pathways: Ubiquitin Proteasome Pathway