Clusterin antibody (AA 150-300)

Catalog No. ABIN7126229

This Mouse Monoclonal antibody specifically detects Clusterin in WB and IHC (f). It exhibits reactivity toward Human.

Quick Overview for Clusterin antibody (AA 150-300) (ABIN7126229)

Target: Clusterin (CLU)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Clusterin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Product Details anti-Clusterin Antibody

Binding Specificity: AA 150-300

Specificity: Clusterin, also designated complement lysis inhibitor (CLI), apolipoprotein J (APOJ), sulfated glycoprotein 2 (SGP2), SP40 and testosterone-repressed prostate message 2 (TRPM2), is a secretory, heterodimeric glycoprotein that influences immune regulation, cell adhesion, transformation, lipid transportation, tissue remodeling, membrane recycling and cell-cell interactions. Clusterin is synthesized as a 449 amino acid polypeptide that is post-translationally cleaved at an internal bond between Arg 227 and Ser 228. Two subunits, i± and i², are associated through disulfide bonds. The i² subunit (also called ApoJi±) corresponds to residues 23-227. The i± subunit (also called ApoJi²) corresponds to residues 228-449. Overexpression of Clusterin appears to be more common in late stages of mammary tumor progression. Clusterin markedly influences i²-Amyloid structure and neuritic toxicity in vivo and may influence Alzheimer s pathogenesis.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment (around aa150-300) of human CLU protein (exact sequence is proprietary)

Isotype: IgG2a

Application Details

Application Notes:

Known_Application: Western Blot (2-4 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Human pancreas, tonsil or cervix.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for Clusterin

Target: Clusterin (CLU)

Alternative Name: CLU

Background: Aging associated protein 4, Apolipoprotein J, CLU1, CLU2, Clusterin alpha / beta chain, Complement associated protein SP40, Complement cytolysis inhibitor a chain, NA1/NA2, D14Ucla3, Dimeric acid glycoprotein, GP80, Ku70-binding protein 1 (KUB1), Sulfated glycoprotein 2 (SGP2), Testosterone repressed prostate message 2 (TPRM2B),Clusterin / Apolipoprotein J (APO-J)
Cellular localisation: Secreted. Can retrotranslocate from secretory compartments to cytosol upon cellular stress and Nucleus. Cytoplasm. Mitochondrion membrane. Cytoplasm, cytosol. Microsome. Endoplasmic reticulum. Cytopla

Molecular Weight: 70kDa (precursor), 36-39kDa (i±), 34-36kDa (i²)

Gene ID: 1191, 436657

UniProt: P10909

Pathways: Apoptosis, Negative Regulation of intrinsic apoptotic Signaling