ZBTB46 antibody

Catalog No. ABIN7126257

The Mouse Monoclonal anti-ZBTB46 antibody has been validated for WB, IF, FACS and IHC (f). It is suitable to detect ZBTB46 in samples from Human.

Quick Overview for ZBTB46 antibody (ABIN7126257)

Target: ZBTB46 (ZNF340) (Zinc Finger Protein 340 (ZNF340))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ZBTB46 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Product Details anti-ZBTB46 Antibody

Specificity: BTBD4 (BTB/POZ domain-containing protein 4), also known as zinc finger protein 340 (ZNF340) or zinc finger and BTB domain-containing protein 46 (ZBTB46), is a 589 amino acid protein that contains one BTB/POZ domain. The BTB/POZ domain mediates homomeric and heteromeric POZ-POZ inter- actions and is common to transcriptional regulators involved in chromatin modeling. In several BTB/POZ containing proteins, including BCL-6 and the promyelocytic leukemia zinc-finger (PLZF) oncoprotein, this domain interacts with the SMRT/N-CoR-mSin3A HDAC complex and is directly involved in repressing and silencing gene transcription. When this domain is deleted, as with the oncogenic PLZF-RAR chimera of promyelocytic leukemias, this tran- scriptional repression is attenuated. This suggests that BTBD4 may play a role in transcription regulation.

Cross-Reactivity (Details): Human. Predicted to react with Mouse and Rat.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human ZBTB46 protein

Isotype: IgG2a

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Human tonsil, brain or rectum. HeLa or U-2 OS cells.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for ZBTB46

Target: ZBTB46 (ZNF340) (Zinc Finger Protein 340 (ZNF340))

Alternative Name: ZBTB46

Background: BTB/POZ domain containing protein 4, BTBD4, RINZF, Zinc finger and BTB domain containing protein 46, Zinc finger protein 340, ZNF340,ZBTB46 / BTBD4 / ZNF340
Cellular localisation: Nucleus

Molecular Weight: 64kDa

Gene ID: 140685, 585028

UniProt: Q86UZ6