CSTB antibody

Catalog No. ABIN7126265

This Mouse Monoclonal antibody specifically detects CSTB in WB and IHC (f). It exhibits reactivity toward Human.

Quick Overview for CSTB antibody (ABIN7126265)

Target: CSTB (Cystatin B (Stefin B) (CSTB))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CSTB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Product Details anti-CSTB Antibody

Specificity: Cystatin A (also designated STF1, STFA, stefin A or cystatin AS) and cystatin B (also designated PME, CST6, STFB, CPI-B, stefin B and liver thiol proteinase inhibitor) are thiol protease inhibitors that form complexes with papain and the cathepsins B, H, and L. cystatin A, a cytoplasmic protein, is one of the precursor proteins of the cornified cell envelope in keratinocytes and plays a role in epidermal development and maintenance. cystatin B protects against intracellular proteases leaking out of lysosomes and is primarily expressed in heart, liver and kidney.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human Cystatin B protein

Isotype: IgG1

Application Details

Application Notes:

Known_Application: Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: HEK293T or U-87 MG cells. Human tonsil.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for CSTB

Target: CSTB (Cystatin B (Stefin B) (CSTB))

Alternative Name: CSTB

Background: CHROW21, CPI-B, CST 6, CSTB, CYTB, Stefin B, EPM1, Liver thiol proteinase inhibitor, PME, STFB, ULD,Cystatin B (Marker of Epithelial Ovarian Cancer Progression)
Cellular localisation: Cytoplasm and nucleus.

Molecular Weight: 12kDa

Gene ID: 1476, 695

UniProt: P04080

Pathways: Response to Water Deprivation