CTNND1 antibody (AA 138-276)

Catalog No. ABIN7126271

This Mouse Monoclonal antibody specifically detects CTNND1 in IHC (f). It exhibits reactivity toward Human.

Quick Overview for CTNND1 antibody (AA 138-276) (ABIN7126271)

Target: CTNND1 (Catenin (Cadherin-Associated Protein), delta 1 (CTNND1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CTNND1 antibody is un-conjugated

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Product Details anti-CTNND1 Antibody

Binding Specificity: AA 138-276

Specificity: Alpha-catenin and beta-catenin bind to the intracellular domain of E-cadherin while p120 catenin binds E-cadherin at a juxta-membrane site. The complex stabilizes tight junctions. In the cell, p120 catenin localized to the E-cadherin/catenins cell adhesion complex, directly associates with cytoplasmic C-terminus of E-cadherin and may similarly interact with other cadherins. p120 is a proliferation-associated nucleolar protein found in most human malignant tumors, but not in resting normal cells. In colorectal cancer the altered localization of p120 catenin corresponds with loss of cytoplasmic localization of E-cadherin. Studies have shown accurate categorization of ductal vs. lobular neoplasia in the breast was achieved with p120 staining. p120 expression further clarifies the separation of low-grade ductal carcinoma in situ from lobular neoplasia. Studies also have shown that altered expression of p120 catenin antibody predicts poor outcome in invasive breast cancer.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment (around aa138-276) of human p120 (exact sequence is proprietary)

Isotype: IgG2b

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Human colon tissue.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for CTNND1

Target: CTNND1 (Catenin (Cadherin-Associated Protein), delta 1 (CTNND1))

Alternative Name: CTNND1

Background: Cadherin-associated Src substrate (CAS), Catenin (cadherin associated protein) delta 1, Catenin delta-1, CTNND1, p120 catenin, P120 CTN, p120(cas), p120(ctn), P120CAS, P120CTN,p120 / Catenin, delta-1 (CTNND1)
Cellular localisation: Cell Surface. Cytoplasm.

Molecular Weight: 120kDa

Gene ID: 1500, 166011

UniProt: O60716

Pathways: EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Cell-Cell Junction Organization, CXCR4-mediated Signaling Events, Platelet-derived growth Factor Receptor Signaling