CD55 antibody (AA 1-100)

Catalog No. ABIN7126277

This Mouse Monoclonal antibody specifically detects CD55 in WB. It exhibits reactivity toward Human.

Quick Overview for CD55 antibody (AA 1-100) (ABIN7126277)

Target: CD55 (Complement Decay-Accelerating Factor (CD55))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD55 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CD55 Antibody

Binding Specificity: AA 1-100

Specificity: Recognizes a single chain glycoprotein of 70 kDa, identified as CD55/DAF. It is widely expressed on cells throughout the body including leukocytes, erythrocytes, epithelium, endothelium, and fibroblasts. It is a Glycosyl phosphatidylinositol anchored (GPI-anchored) member of the membrane bound complement regulatory proteins that inhibit autologous complement cascade activation. It prevents the amplification steps of the complement cascade by interfering with the assembly of the C3-convertases, C4b2a and C3bBb, and the C5-convertase, C4b2a3b and C3bBb3b. CD55 also serves as receptor for CD97 and for echovirus and Coxsackie B virus. The MAb 143-30 may be used as marker for paroxysmal nocturnal hemoglobinuria (PNH).

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment (around aa1-100) of human CD55 (exact sequence is proprietary)

Isotype: IgG1

Application Details

Application Notes:

Known_Application: Western Blot (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.

Positive_Control: Jurkat cells or human lymphocytes. Human lymph nodes and tonsils.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for CD55

Target: CD55 (Complement Decay-Accelerating Factor (CD55))

Alternative Name: CD55

Background: CD55, CD55 Cromer blood group system, Complement decay-accelerating factor, CROM, DAF, GPI-DAF, Decay Accelerating Factor for Complement, Decay accelerating factor GPI-form, Decay accelerating factor soluble-form,CD55 / Decay Accelerating Factor (DAF)
Cellular localisation: Cell Surface. Secreted.

Molecular Weight: 70kDa

Gene ID: 1604, 126517

UniProt: P08174

Pathways: Complement System, Regulation of Leukocyte Mediated Immunity