Decorin antibody (C-Term)

Catalog No. ABIN7126284

The Mouse Monoclonal anti-Decorin antibody has been validated for WB, IHC, IF, FACS and IHC (f). It is suitable to detect Decorin in samples from Cow.

Quick Overview for Decorin antibody (C-Term) (ABIN7126284)

Target: Decorin (DCN)

Reactivity: Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This Decorin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Product Details anti-Decorin Antibody

Binding Specificity: C-Term

Specificity: Decorin is a small leucine-rich proteoglycan (SLRP) family member that consists of a glycosaminoglycan chain-containing core protein. The core protein contains ten leucine rich repeats that contain sites for glycosylation, flanked by disulfide bond stabilizing loops. Decorin binds to Collagen Type I, II and IV in vivo and promotes the formation of fibers with variations in stability and solubility. The Decorin core protein binds to growth factors, intercellular matrix molecules, such as Fibronectin and Thrombospondin, and to the Decorin endocytosis receptor. Decorin binds to and inhibits TGFi² and is a direct or indirect negative modulator of TGFi² synthesis. Inhibition of Decorin core protein gene expression by the combination of IFN-i³ and TNFi± may contribute to cartilage destruction that is characteristic of inflammatory joint diseases. For immunostaining, pre-incubation with chondroitinase-SBC or testicular hyaluronidase may be required to expose the epitope.

Cross-Reactivity (Details): Human, Bovine, Canine, Chick, Elk, Equine, Mouse, Porcine and Rabbit.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length bovine DCN protein corresponding to C-terminal core

Isotype: IgG1

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Human prostate or skin tissue (IHC).

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for Decorin

Target: Decorin (DCN)

Alternative Name: DCN

Background: Bone proteoglycan II, CSCD, Dermatan sulphate proteoglycans II (DSPG2), PG40, PGII, PGS2, Proteoglycan core protein, SLRR1B, Small leucine rich protein 1B,Decorin
Cellular localisation: Secreted.

Molecular Weight: 43kDa

Gene ID: 1634, 728830

UniProt: P07585

Pathways: Glycosaminoglycan Metabolic Process