IDO2 antibody (AA 200-350)

Catalog No. ABIN7126294

Product Details anti-IDO2 Antibody

Target: IDO2 (Indoleamine 2,3-Dioxygenase 2 (IDO2))

Binding Specificity: AA 200-350

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IDO2 antibody is un-conjugated

Application: ELISA, Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificity: IDO2 is a presumptive immunomodulatory gene based on its close structural relationship to IDO1 and its expression in a variety of antigen-presenting cell types. Both IDO1 and IDO2 will catabolize tryptophan to kynurenine. Biochemical studies indicate that both enzymes are similarly robust in catabolic activity, although the in vitro conditions required for IDO2 to manifest the same level of activity differ somewhat from IDO1. However, whether IDO2 is active as a tryptophan catabolizing enzyme in human dendritic cells has been disputed. Further work is needed to conclusively determine that IDO1 and IDO2 are similar in their preference for substrates and reaction pathways to generate product. A non-redundant function for IDO2 relative to IDO1 is suggested by their rather distinct expression patterns and response to extracellular stimuli.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment (around aa 200-350) of human IDO2 protein (exact sequence is proprietary)

Isotype: IgG

Application Details

Application Notes:

Known_Application: ELISA (For coating, order antibody without BSA),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Human placenta or liver.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for IDO2

Target: IDO2 (Indoleamine 2,3-Dioxygenase 2 (IDO2))

Alternative Name: IDO2 (IDO2 Products)

Background: 3-dioxygenase 2, 3-dioxygenase-like protein 1, EC 1.13.11., INDOL 1, Indoleamine 2, Indoleamine 2,3 dioxygenase 2, Indoleamine 2,3 dioxygenase like 1 protein, Indoleamine pyrrole 2,3 dioxygenase like 1, Indoleamine-pyrrole 2,IDO2 / Indoleamine 2,3-dioxygenase 2
Cellular localisation: Cytoplasm

Molecular Weight: 47kDa

Gene ID: 169355, 676257

UniProt: Q6ZQW0