EIF4A2 antibody

Catalog No. ABIN7126324

This anti-EIF4A2 antibody is a Mouse Monoclonal antibody detecting EIF4A2 in ELISA and Coat. Suitable for Human.

Quick Overview for EIF4A2 antibody (ABIN7126324)

Target: EIF4A2 (Eukaryotic Translation Initiation Factor 4A2 (EIF4A2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This EIF4A2 antibody is un-conjugated

Application: ELISA, Coating (Coat)

Product Details anti-EIF4A2 Antibody

Specificity: Translation initiation in eukaryotes necessitates the assembly of an 80S ribosomal complex. Eukaryotic initiation factors (eIFs) are utilized in a sequence of reactions that leads to 80S ribosomal assembly and initiation of translation. Mammalian eukaryotic translation initiation factor 4F (eIF4F) is a protein complex that contains eIF4A, eIF4E and eIF4G, binds mRNA at a5'-cap motif and recruits the 43S ribosomal preinitiation complex to the eligible transcript. Along with eIF4B, the eIF4F complex mediates the unwinding of mRNA secondary structure to facilitate ribosome association. eIF4E specifically interacts with the 5' cap, eIF4A (I, II) are bidirectional RNA helicases, and eIF4G (I, II) are scaffolding proteins which coordinate eIF4E, eIF4A, eIF3and the 40S ribosome. Human eIF4AI (eIF4A, DDX2A) is a 406 amino acid protein that is 92.7% homologous to mouse eIF4AI. The promoter region of human eIF4A1 contains TATA and CAAT motifs and consensus binding sites to Sp1 and AP2.

Cross-Reactivity (Details): Human. Predicted to work in Chick, Mouse, Rat, Xenopus and Zebrafish.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human EIF4A2 protein

Isotype: IgG2a

Application Details

Application Notes:

Known_Application: ELISA (For coating, order Ab without BSA),Optimal dilution for a specific application should be determined.

Positive_Control: HeLa cells.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for EIF4A2

Target: EIF4A2 (Eukaryotic Translation Initiation Factor 4A2 (EIF4A2))

Alternative Name: EIF4A2

Background: ATP dependent RNA helicase eIF4A 2, BM-010, DDX2B, EIF4F, eukaryotic translation initiation factor 4A isoform 2, N-terminally processed,EIF4A2 (Eukaryotic Initiation Factor 4A-II)
Cellular localisation: Nucleus.

Molecular Weight: 46kDa

Gene ID: 1974

UniProt: Q14240