alpha 2 Macroglobulin antibody (AA 604-748)

Catalog No. ABIN7126327

This anti-alpha 2 Macroglobulin antibody is a Mouse Monoclonal antibody detecting alpha 2 Macroglobulin in IHC (f). Suitable for Human.

Quick Overview for alpha 2 Macroglobulin antibody (AA 604-748) (ABIN7126327)

Target: alpha 2 Macroglobulin (A2M) (alpha-2-Macroglobulin (A2M))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This alpha 2 Macroglobulin antibody is un-conjugated

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Product Details anti-alpha 2 Macroglobulin Antibody

Binding Specificity: AA 604-748

Specificity: I±-2-Macroglobulin (i±-2M) is a homotetrameric serum protein consisting of four identical subunits that form dimers through disulfide bonds. Initially, i±-2M was characterized as a pan-proteinase inhibitor that was able to bait proteinases into cleaving specific peptide sequences on i±-2M. This interaction induces a conformational change in i±-2M, thus enabling it to trap the proteinase and further inhibit its activity. Subsequently, i±-2M has been shown to function as a carrier protein and regulator of cytokines during inflammation. Circulating transforming growth factor i² (TGFi²) in serum is primarily bound to i±-2M, which renders TGFi² inactive. i±-2M also binds to IL-6 and, thereby, increases the concentration of IL-6 near lymphocytes, hepatocytes and stem cells involved in mediating the inflammatory cascade. Mutations and deletions in the gene encoding i±-2M are associated with an increased incidence of Alzheimer s disease (AD), which is consistent with the role of i±-2M in mediating the clearance and degradation of A i², the major component of i²-Amyloid deposits accumulated during AD.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Human recombinant A2M protein fragment (around aa 604-748) (exact sequence is proprietary)

Isotype: IgG2b

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Human liver or placenta tissues.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for alpha 2 Macroglobulin

Target: alpha 2 Macroglobulin (A2M) (alpha-2-Macroglobulin (A2M))

Alternative Name: A2M

Background: C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5, Alpha-2-M (A2M), CPAMD5,Alpha-2-Macroglobulin
Cellular localisation: Secreted in plasma

Molecular Weight: 185kDa

Gene ID: 2, 212838

UniProt: P01023

Pathways: Lipid Metabolism