ESR2 antibody (C-Term)

Catalog No. ABIN7126353

The Mouse Monoclonal anti-ESR2 antibody has been validated for WB, IF, FACS and IHC (f). It is suitable to detect ESR2 in samples from Human.

Quick Overview for ESR2 antibody (C-Term) (ABIN7126353)

Target: ESR2 (Estrogen Receptor 2 (ESR2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ESR2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Product Details anti-ESR2 Antibody

Binding Specificity: C-Term

Specificity: This MAb recognizes the estrogen receptor beta 2 isoform, expressed in cell nuclei of a range of human tissues including ovary, placenta, testis and vas deferens. No cross-reactivity is seen with estrogen receptor beta 1 isoform. This gene encodes a member of the family of estrogen receptors and superfamily of nuclear receptor transcription factors. The gene product contains an N-terminal DNA binding domain and C-terminal ligand binding domain and is localized to the nucleus, cytoplasm, and mitochondria. Upon binding to 17beta-estradiol or related ligands, the encoded protein forms homo- or hetero-dimers that interact with specific DNA sequences to activate transcription. Some isoforms dominantly inhibit the activity of other estrogen receptor family members.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: A synthetic peptide (CGMKMETLLPEATMEQ ) from the C terminus of the human estrogen receptor beta 2 isomer

Isotype: IgG1

Application Details

Application Notes:

Known_Application: Flow Cytometry (2-4 μg/million cells), Immunofluorescence (2-4 μg/mL), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: MCF-7 cells. Human testis tissue.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for ESR2

Target: ESR2 (Estrogen Receptor 2 (ESR2))

Alternative Name: ESR2

Background: Estrogen Receptor 2, Nuclear Receptor Subfamily 3 Group A Member 2 (NR3A2), Oestrogen Receptor Beta, Estrogen Receptor Beta, ER-BETA, ESTRB,ER-beta (Estrogen Receptor beta-2)
Cellular localisation: Nuclear

Molecular Weight: 59kDa

Gene ID: 2100, 660607

UniProt: Q92731

Pathways: Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling