JAZF1 antibody (AA 1-130)

Catalog No. ABIN7126392

The Mouse Monoclonal anti-JAZF1 antibody has been validated for WB, FACS, IF, IHC (f) and IP. It is suitable to detect JAZF1 in samples from Human.

Quick Overview for JAZF1 antibody (AA 1-130) (ABIN7126392)

Target: JAZF1 (JAZF Zinc Finger 1 (JAZF1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This JAZF1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunoprecipitation (IP)

Product Details anti-JAZF1 Antibody

Binding Specificity: AA 1-130

Specificity: JAZF1 (juxtaposed with another zinc finger protein 1), also known as TIP27 (TAK1-interacting protein 27) or ZNF802 (zinc finger protein 802), is a 243 amino acid protein that localizes to the nucleus and contains three C2H2-type zinc fingers. Existing as multiple alternatively spliced isoforms, JAZF1 interacts with the nuclear orphan receptor TR4 and is thought to function as a transcriptional repressor, effectively down-regulation the expression of TR4. Chromosomal aberrations in the gene encoding JAZF1 are associated with the pathogenesis of endometrial stromal tumors, suggesting a role for JAZF1 in carcinogenesis.

Cross-Reactivity (Details): Human. Predicted to react in Mouse.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment (around aa1-130) of human JAZF1 protein (exact sequence is proprietary)

Isotype: IgG2a

Application Details

Application Notes:

Known_Application: Immunoprecipitation (1-2 μg per 100-500 μg of total protein), Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: HeLa or U87 cells. Ubiquitous tissue expression.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for JAZF1

Target: JAZF1 (JAZF Zinc Finger 1 (JAZF1))

Alternative Name: JAZF1

Background: DKFZp761K2222, TIP27, ZNF802,JAZF1 (Transcription Corepressor)
Cellular localisation: Nucleus.

Molecular Weight: 27.08kDa

Gene ID: 221895, 368944

UniProt: Q86VZ6