FOXL1 antibody (AA 104-258)

Catalog No. ABIN7126396

This anti-FOXL1 antibody is a Mouse Monoclonal antibody detecting FOXL1 in WB, IF, FACS, IHC (f) and IP. Suitable for Human.

Quick Overview for FOXL1 antibody (AA 104-258) (ABIN7126396)

Target: FOXL1 (Forkhead Box L1 (FOXL1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This FOXL1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunoprecipitation (IP)

Product Details anti-FOXL1 Antibody

Binding Specificity: AA 104-258

Specificity: FOXL1 is a 337 amino acid protein encoded by the mouse gene FOXL1. FOXL1 belongs to the forkhead family and contains one forkhead DNA-binding domain. The HNF3/forkhead family includes a large number of transcription factors that share a structurally related DNA binding domain. forkhead factors are known to play important roles both during development and in adults. FOXL1 is a winged helix transcriptional regulator expressed in the mesenchymal layer of developing and mature gastrointestinal tract. FOXL1-deficient mice exhibit various defects not only in the epithelial layer of the gastrointestinal tract but also in gut-associated lymphoid tissues. In the small intestine of FOXL1-deficient mice, the formation of Peyer's patches is affected, particularly in the caudal region. FOXL1 is a mesenchymal modifier of the Adenomatous Polyposis Coli (APC) gene products and plays a key role in gastrointestinal tumorigenesis.

Cross-Reactivity (Details): Human. Predicted to react in Rat.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment (around aa104-258) of human FOXL1 protein (exact sequence is proprietary)

Isotype: IgG1

Application Details

Application Notes:

Known_Application: Immunoprecipitation (1-2 μg per 100-500 μg of total protein), Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: HeLa or U87 cells. Ubiquitous tissue expression.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for FOXL1

Target: FOXL1 (Forkhead Box L1 (FOXL1))

Alternative Name: FOXL1

Background: FKHL10, FREAC6, JFJ-3, FKH6, FKH11, Forkhead box protein L1, Forkhead related transcription factor, FREAC7,FOXL1 / Forkhead Box Protein L1 (Transcription Factor)
Cellular localisation: Nucleus.

Molecular Weight: 36-41kDa

Gene ID: 2299, 87236

UniProt: Q12951