ZHX3 antibody

Catalog No. ABIN7126398

The Mouse Monoclonal anti-ZHX3 antibody has been validated for WB, ELISA, IF, IP, Coat, FACS and IHC (f). It is suitable to detect ZHX3 in samples from Human.

Quick Overview for ZHX3 antibody (ABIN7126398)

Target: ZHX3 (Zinc Fingers and Homeoboxes 3 (ZHX3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This ZHX3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Coating (Coat), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Product Details anti-ZHX3 Antibody

Specificity: Zinc-fingers and homeobox (ZHX) proteins are transcription factors that interact with the activation domain of the A subunit of nuclear factor-Y (NF-YA). ZHX1-3 are ubiquitously expressed proteins expressed in various tissues. They act as transcriptional repressors and localize to the nucleus. The ZHX proteins contain two Cys2-His2-type zinc-finger motifs and five homeodomains (HDs). These domains allow the ZHX proteins to form homodimers, but they can also form heterodimers with each other. However, this dimerization is not required for repressor activity. Hypermethylation-mediated silencing of ZHX2 is an epigenetic event involved in hepatocellular carcinoma (HCC).

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human ZHX3 protein

Isotype: IgG2a

Application Details

Application Notes:

Known_Application: ELISA (For coating, order antibody without BSA), ,Immunoprecipitation (1-2 μg per 100-500 μg of total protein (1 mL of cell lysate)), ,Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Human kidney, brain or thyroid.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for ZHX3

Target: ZHX3 (Zinc Fingers and Homeoboxes 3 (ZHX3))

Alternative Name: ZHX3

Background: KIAA0395, TIX1, Triple homeobox protein 1, ZHX 3, Zhx3, Zinc finger and homeodomain protein 3, Zinc fingers and homeoboxes protein 3,ZHX3 (Transcriptional Repressor)
Cellular localisation: Nucleus.

Molecular Weight: 105kDa

Gene ID: 23051, 380133

UniProt: Q9H4I2